NaCl胁迫对不同来源木豆种子发芽及幼苗生长的影响

Effects of NaCl Stress on Seed Germination and Seedling Growth of Pigeonpea Accessions from Different Origins

为研究NaCl胁迫对木豆种子发芽及幼苗生长的影响,采用不同质量分数NaCl溶液对5份分别来源于印度(YD1)、缅甸(MD1)、云南(YN1)、广西(GX1)和广东(GD1)的木豆种子和幼苗进行胁迫处理。分别采用培养皿发芽法和水培法,设置不同质量分数的NaCl处理,通过观测木豆种子萌发特性和幼苗生长状况,分析NaCl胁迫对木豆种子萌发和幼苗生长的影响,并通过隶属函数分析对木豆耐盐性进行综合评价,计算木豆NaCl胁迫的临界值。结果表明:在萌发期,NaCl胁迫抑制种子萌发及生长,5份木豆种质资源耐盐能力强弱次序为MD1>GX1>YD1>GD1>YN1。幼苗期,随NaCl质量分数增大,木豆幼苗地上及地下部生长均被显著抑制,5份木豆耐盐能力强弱次序为MD1>GX1>GD1>YD1>YN1。在种子萌发期和幼苗期,5份不同木豆耐盐能力强弱次序并不完全一致,MD1和GX1为耐盐种质,YN1为盐敏感种质。萌发期和幼苗期,5份木豆NaCl胁迫临界值范围分别为0.377%~0.748%和0.275%~0.510%。

An attempt was made to analyze the effect of salt stress on seed germination and seedling growth of pigeonpea(Cajanus cajan).Seeds and seedlings of 5 pigeonpea accessions collected from India(YD1),Myanmar(MD1),Yunnan(YN1),Guangxi(GX1)and Guangdong(GD1)were treated with NaCl solution at different concentrations to observe their seed germination and seedling growth,and theirThe salt tolerance was evaluated by membership function method.The critical concentrations of NaCl stress for different pigeonpea accessions were determined at the germination and seedling stages.The seed germination and growth were found to be inhibited by NaCl stress at the germination stage,and the salt tolerance of 5 different pigeonpea accessions at the germination stage was in the order of MD1>GX1>YD1>GD1>YN1.The aboveground and underground growth of pigeonpea seedlings were significantly inhibited with the increase of NaCl concentration at the seedling stage,and the salt tolerance of 5 different pigeonpea accessions at the seedling stage was in the order of MD1>GX1>GD1>YD1>YN1.The salt tolerance of different pigeonpea accessions at the seed germination stage was not completely consistent with that at the seedling stage.The results showed that MD1 and GX1 were salt-tolerant while YN1 was salt-sensitive.The critical NaCl concentrations for the 5 pigeonpea accessions at the germination and seedling stages were 0.377%−0.748%and 0.275%−0.510%,respectively.This might provide an important information for screening and evaluation of pigeonpea germplasm and breeding of new salt-tolerant pigeonpea varieties.