超声辅助提取川产木香中木香烃内酯和去氢木香内酯的研究

Study on Ultrasonic-assisted Extraction of Costundide and Dehydrocostus Lactone from Aucklandia costus Falc.Produced in Sichuan Province

以川产木香的根茎为原料、柠檬烯为提取溶剂,采用超声细胞破碎辅助提取木香烃内酯和去氢木香内酯。以高效液相色谱法为定量手段,考察了液固比、超声功率、空化时间、缓冲时间、超声时间对木香烃内酯和去氢木香内酯得率的影响,得到最佳提取条件分别为,在超声功率350 W和超声时间40 min的条件下,溶剂柠檬烯与木香的比率为25 mL·g^(−1),其空化时间1.5 s、缓冲时间2.0 s。超声细胞破碎辅助提取法具有高效、节能、绿色环保的优点。

Using the rhizomes of Aucklandia costus Falc.produced in Sichuan Province as raw materials and limonene as extraction solvent,costunolide and dehydrocostus lactone were extracted by ultrasonic cell disruption assisted extraction method.Effects of liquid-solid ratio,ultrasonic power,cavitation time,buffer time,and ultrasonic time on the yield of costunolide and dehydrocostus lactone were investigated by the quantitative method of high performance liquid chromatography.The best extraction conditions were as follows:under the ultrasonic power 350 W,ultrasonic time 40 min,the ratio of limonene to Radix Aucklandiae was 25 mL·g^(−1),the cavitation time was 1.5 s,and the buffer time was 2.0 s.Ultrasonic cell disruption assisted extraction method had the advantages of high efficiency,energy saving,and environmental protection.