AIF和BNIP3在血清剥夺诱导的PC12细胞损伤和死亡过程中的变化

Profiles of AIF and BNIP3 in process of PC12 cell injury and death induced by serum deprivation

目的:研究凋亡诱导因子(AIF)和促凋亡调节蛋白3(BNIP3)在血清剥夺诱导的PC12细胞损伤和死亡过程中的变化。方法:PC12细胞在不含10%胎牛血清的DMEM培养基中培养,建立PC12血清剥夺模型,观察其增殖情况以及AIF和BNIP3的表达情况。结果:随血清剥夺的时间增加,细胞死亡率上升(P<0.05)。细胞免疫荧光实验显示,血清剥夺后AIF在细胞核中分布增多,胞核形状不一并聚集成团。免疫印迹法进一步证实了血清剥夺时间越久,AIF和BNIP3在细胞和线粒体中表达水平越高(P<0.05)。结论:AIF与BNIP3在血清剥夺诱导的细胞损伤和死亡相关过程中表达水平上升。

Objective:To investigate the profiles of apoptosis-inducing factor(AIF)and Bcl-2 interacting protein 3(BNIP3)in the process of PC12 cell injury and death induced by serum deprivation.Methods:PC12 cells were cultured in DMEM medium without 10%fetal bovine serum,thereby a serum deprivation model of PC12 was established.The proliferation of PC12 and the expression of AIF and BNIP3 were examined.Results:Along with longer duration of serum deprivation,the cell death rate increased(P<0.05).Immunofluorescence experiments using PC12 cells showed that after serum deprivation,there was an increase in distribution of AIF in the cell nuclei which appeared shape-incongruent and aggregated.Western blotting confirmed that longer duration of serum deprivation was assocated with higher expression levels of AIF and BNIP3 in PC12 cells and mitochondria(P<0.05).Conclusion:The expression of AIF and BNIP3 is upregulated in process of cell injury and death induced by serum deprivation.