摘要
Objective To observe the therapeutic effect of mild moxibustion on irritable bowel syndrome(IBS)visceral hyperalgesia model rats and its regulatory effect on P2X3 receptors in the spinal cord,anterior cingutate cortex(ACC)and thalamic ventral posterolateral nucleus(VPL).Methods Thirty 8-day-old newborn rats were randomly divided into a normal group(n=6)and a modeling group(n=24)according to the completely random number table method.Rats in the normal group were bred routinely,and those in the modeling group were subjected to preparing IBS chronic visceral hyperalgesia model using colorectal distention(CRD)in stimulation method.Rats successfully modelled were re-divided into a model group,a mild moxibustion group,a P2X3 receptor antagonist group,and a normal saline group according to the completely random number table method with 6 rats in each group.Rats in each group received corresponding interventions from the 37-day old,once a day for 7 consecutive days.Immunohistochemistry and Western blot assays were used to detect P2X3 protein expressions in the spinal cord,ACC and VPL of rats.Results Under different intensities of CRD stimulation,the abdominal withdrawal reflex(AWR)scores of the model group were significantly increased versus the normal group(all P<0.05);the AWR scores of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group(all P<0.01).The P2X3 protein expressions in rat spinal cord,ACC and VPL tissues of the model group were significantly increased versus the normal group(all P<0.01);the P2X3 protein expressions in rat spinal cord,ACC and VPL tissues of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group(all P<0.01).Conclusion Mild moxibustion can inhibit the P2X3 receptor expressions in the spinal cord,ACC,and VPL tissues of IBS visceral hyperalgesia model rats,which may be the mechanism of mild moxibustion in relieving the central sensitization of rats with IBS visceral hyperalgesia.
目的观察温和灸对肠易激综合征(IBS)内脏痛敏模型大鼠的干预作用及其对脊髓、前扣带回(ACC)和丘脑腹后外侧核(VPL)中P2X3受体的调节作用.方法按照完全随机数字表法将30只8日龄新生大鼠随机分为正常组(n=6)与造模组(n=24).正常组常规饲养,造模组采用直结肠球囊扩张(CRD)刺激法制备IBS慢性内脏痛敏大鼠模型.将模型验证成功的造模大鼠按照完全随机数字表法重新分为模型组、温和灸组、P2X3受体拮抗剂组和生理盐水组,每组6只.各组大鼠从37日龄开始分别接受相应的干预,每日1次,连续7 d.采用免疫组织化学法及免疫印迹法检测大鼠脊髓、ACC和VPL内P2X3蛋白表达.结果在不同强度的CRD刺激下,与正常组相比,模型组AWR评分均明显升高(均P<0.05);与模型组相比,温和灸组及P2X3受体拮抗剂组AWR评分均显著降低(均P<0.01).与正常组相比,模型组大鼠脊髓、ACC及VPL组织中P2X3蛋白的表达均显著升高(均P<0.01);与模型组相比,温和灸组及P2X3受体拮抗剂组大鼠脊髓、ACC及VPL组织中P2X3蛋白的表达均显著降低(均P<0.01).结论温和灸能够抑制IBS内脏痛敏模型大鼠脊髓、ACC及VPL组织内P2X3受体的表达,这可能是温和灸缓解IBS内脏痛敏大鼠中枢敏化的效应机制.
基金
上海市卫生和计划生育委员会科研课题,No.20174Y0015
国家自然科学基金项目,No.81904301
973 Program,国家重点基础研究发展计划项目No.2015CB554501
上海中医药事业发展三年行动计划项目,No.ZY(2018–2020)-CCCX-2004-01
国家中医药领军人才支持计划-岐黄学者.
