四川茂县花椒根腐病病原菌的分离与鉴定

The Pathogen Causing Root Rot in Zanthoxylum bungeanum in Mao County of Sichuan Province:Isolation and Identification

旨在明确引起阿坝藏族羌族自治州茂县花椒根腐病的病原菌种类,为科学有效防治该病提供理论参考。以采自茂县的花椒根腐病病根为材料,通过组织分离法、致病性测定、形态学观察与基于核糖体转录间隔区序列(ITS)和延长因子基因(tef1)的分子鉴定相结合进行病原菌的分离鉴定。结果表明,病原菌菌丝初为灰白色,后为兰褐色或紫红色。大型分生孢子为镰刀形,小型分生孢子呈肾形。分子鉴定结果显示,该病原菌在基于ITS和tef1序列构建的系统发育进化树上与腐皮镰孢菌类群处于同一分支。根据形态学和分子鉴定结果,将引起四川茂县花椒根腐病的病原菌鉴定为腐皮镰孢菌(Fusarium solani)。

To identify the pathogen species causing root rot in Zanthoxylum bungeanum in Mao County,Aba Tibetan and Qiang Autonomous Prefecture,and provide theoretical reference for scientific and effective treatment of this disease,the rot roots of Z.bungeanum were collected from Mao County and the pathogen was isolated and identified by tissue isolation method,pathogenicity determination,morphological characteristics and molecular identification which was based on ITS and tef1 gene sequences.The results showed that the mycelium of pathogen was gray white at the beginning,and then became blue brown or purple red.Large conidia of the pathogen were sickle-shaped and small conidia were kidney-shaped.Phylogenetic analysis based on ITS and tef1 gene sequences showed that the pathogen was attributed to the same branch with Fusarium solani in the phylogenetic tree.According to morphology and molecular identification,the pathogen of root rot in Z.bungeanum in Mao County of Sichuan Province was identified as F.solani.