微塑料和全氟辛烷磺酸类物质共暴露对翡翠贻贝滤食率和抗氧化系统的影响

Effects of Microplastics and PFOS Co-exposure on the Filtration Rate and Antioxidant System of Perna viridis

为了探索微塑料和全氟辛烷磺酸类物质(PFOS)在海洋生物体内的复合污染毒性机制,以翡翠贻贝为研究对象,在聚苯乙烯微塑料(0.2μm)4.55×10^(8)个/L以及PFOS浓度为10,100和1000μg/L的条件下,研究PFOS或微塑料单独暴露以及二者复合暴露对翡翠贻贝滤食率、活性氧水平(ROS)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、谷胱甘肽巯基转移酶(GST)活性和谷胱甘肽还原酶(GR)活性的影响。结果表明,与溶剂对照组相比,暴露于微塑料和PFOS后翡翠贻贝的滤食率均没有显著变化。在翡翠贻贝的鳃、内脏团和性腺中,微塑料或PFOS的胁迫都会引起酶响应的显著变化。与PFOS单独暴露组相比,微塑料和1000μg/L PFOS共同暴露时,ROS水平在鳃和性腺中显著提升,在内脏团中显著下降;MDA含量在鳃和内脏团中显著提升,在性腺中显著下降;GST和GR活性在鳃中显著下降,在性腺中显著提高。研究结果说明微塑料会改变翡翠贻贝对PFOS的氧化应激响应。

In order to explore the composite pollution toxicity mechanism of microplastics and perfluorooctane sulfonate(PFOS)in Perna viridis,the effects of PFOS(10,100,1000μg/L)and microplastics(0.2μm,4.55×10^(8)numbers/L)were studied under single-or co-exposure on the filtration rate,reactive oxygen level(ROS),malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,catalase(CAT)activity,glutathione S-transferase(GST)activity and gluathione reductase(GR)activity.The results showed that the filtration rate of mussels after exposure to microplastics and PFOS did not change significantly compared to the solvent control.In gills,visceral mass and gonads of mussels,the stress of microplastics and PFOS could cause significant changes in enzyme response.After co-exposure of microplastics and 1000μg/L PFOS,compared with single-PFOS exposure groups,ROS level significantly increased in gills and gonads,whereas decreased in visceral mass;MDA content increased in gills and visceral mass,whereas decreased in gonads;GST and GR activity was significantly reduced in gills,whereas increased in gonads.The results indicated that microplastics could change the oxidative stress response of mussels to PFOS.