不同商品规格三七止血功效的谱效关系研究

Spectrum-effect relationship of hemostatic effects of Notoginseng Radix et Rhizoma with different commodity specifications

该文旨在建立不同商品规格三七的化学成分药物谱,测定12个不同商品规格三七的止血药效学指标,结合谱-效相关分析,探讨不同商品规格三七化学成分与止血功效的谱效关系。首先采用HPLC以及液质联用技术建立三七的化学成分药物谱;利用大鼠血浆复钙时间测定实验和大鼠胃出血实验,测定不同商品规格三七的止血药效学指标,包括血浆复钙时间(PRT)、血浆凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血酶时间(APTT);之后再利用偏最小二乘法,探索不同商品规格三七化学成分与止血药效指标间的谱效关系。结果显示,在构建了不同商品规格三七的HPLC指纹图谱基础上,确定了26个共有峰,并指认了其中的11个共有峰,均为皂苷类成分;采用液质联用法测定了不同商品规格三七中三七素含量;大鼠血浆复钙时间测定实验和大鼠胃出血实验均表明不同商品规格三七止血药效指标之间具有明显的差异;利用谱-效相关分析探讨了不同商品规格三七27个共有成分和止血药效指标之间的关系,其中16个成分对三七止血药效指标起正向作用,11个起负向作用,这为三七的临床精准用药及质量控制研究提供了一定的参考依据。

This article aims to establish the fingerprints, determine the hemostatic pharmacodynamic indicators, and explore the spectrum-effect relationship of Notoginseng Radix et Rhizoma in 12 different specifications. Firstly, HPLC and liquid chromatography-mass spectrometry(LC-MS) were employed to establish the fingerprints of Notoginseng Radix et Rhizoma. The rat plasma recalcification experiment and the rat gastric bleeding experiment were conducted to determine the pharmacodynamic indicators, including plasma recalcification time(PRT), thrombin time(TT), prothrombin time(PT), and activated partial thromboplastin time(APTT). Afterwards, the partial least squares method was employed to explore the spectrum-effect relationship of Notoginseng Radix et Rhizoma in different specifications. Twenty-six common peaks were detected in the HPLC fingerprints of different specifications of Notoginseng Radix et Rhizoma, and 11 out of the 26 common peaks represented saponins. The content of dencichine was determined by LC-MS. The rat experiments showed that the pharmacodynamic indicators were significantly different among different specifications of Notoginseng Radix et Rhizoma. The spectrum-effect relationship was explored between 27 common components and pharmacodynamic indicators. Among them, 16 components had positive effects on the pharmacodynamic indicators of Notoginseng Radix et Rhizoma, and 11 exerted negative effects. This study provides a basis for the precision medication and quality control of Notoginseng Radix et Rhizoma.