沙柳SpsNAC042基因克隆及逆境表达分析

Cloning of SpsNAC042 and Its Expression Analysis under Stress in Salix psammophila

采用同源克隆的方法获得沙柳SpsNAC042基因,通过生物信息学分析、组织特异性表达分析、逆境表达分析。结果表明,SpsNAC042基因的开放阅读框序列长度为900 bp,共编码299个氨基酸,测定为亲水性不稳定蛋白。组织特异性表达分析得出花中的基因相对表达量最高,幼叶、茎9~15节间、根、成熟叶的表达量依次降低,茎尖的表达量最低。SpsNAC042基因在干旱处理下与对照组相比整体呈增长趋势,并在8 h处达到最高;在低温处理下,基因表达量迅速积累,在2 h处达到最高,然后维持在较低水平表达;在盐和高温处理下SpsNAC042基因缓慢积累,后期迅速增高,最终在24 h处达到最高。研究表明SpsNAC042基因参与逆境应答反应,为深入开展沙柳NAC基因对生物和非生物胁迫应答研究提供了参考价值。

In this study, SpsNAC042 gene of Salix psammophila was obtained by homologous cloning method.The analyses of bioinformatics, tissue-specific expression, and stress expression indicated that the open reading frame(ORF) sequence of the gene was 900 bp, encoded with 299 amino acids.It was identified as an unstable protein with hydrophilic property.The tissue specific expression analysis showed that the relative expression of the gene in flowers was the highest, and decreased systematically in young leaf, the 9-15th internode in stem, root and mature leaf, the lowest expression was in the shoot apex.The expression increased under drought stress compared with the control group, and reached the highest level at 8 hours.Under low temperature treatment, the gene expression rapidly accumulated and reached the highest level in 2 hours after the treatment, and remained at a low level.SpsNAC042 gene accumulated slowly under salt and high temperature treatments, and increased rapidly in the late stage, finally reached its maximum after 24 hours.The results showed that SpsNAC042 gene was involved in stress response.This study provides a reference for further research on the biological and abiotic stress responses of S.psammophila NAC gene.