肉制品中多组分食品添加剂快速检测的前处理优化研究

Optimization of Pretreatment for Rapid Detection of Multi-Component Food Additives in Meat Products

目的:优化肉制品中多组分食品添加剂快速检测方法的前处理步骤,提高检测结果的准确性。方法:考察样品前处理中的提取溶剂、净化方式、基质效应、滤膜吸附等方式对各组分提取效果的影响。结果:优化后的前处理方法经上机测定,41种食品添加剂在一定质量浓度范围内线性良好,线性相关系数大于0.990。空白样品添加的回收率为64.84%~111.15%,相对标准偏差为1.21%~9.88%(n=6)。结论:优化后的样品前处理方法,目标物分离效果好,线性、回收率与重复性均良好,结果准确性高,适用于大批量肉制品中多组分食品添加剂及非法添加物的快速检测,提高监管效率。

Objective:To optimize the pre-processing steps of the rapid detection method of multi-component food additives in meat products to improve the accuracy of the detection results.Methods:The influence of extraction solvent,purification method,matrix effect,filter membrane adsorption and other methods in sample pretreatment on the extraction effect of each component were investigated.Results:41 kinds of food additives optimized pretreatment method have good linearity within a certain mass concentration range,and the linear correlation coefficient was greater than 0.990.The recovery rate of blank sample addition were between 64.84% and 111.15%,and the relative standard deviation were between 1.21% and 9.88% (n=6).Conclusion:The optimized sample pretreatment method has good separation,linearity,recovery and repeatability,and high accuracy.It was suitable for the rapid detection of multi-component food additives and illegal additives in large quantities of meat products to improve the supervision efficiency.