miR-181b在胃癌患者血清中的水平变化及临床意义

The level of miR-181b in the serum of patients with gastric cancer and its clinical significance

目的探讨微小RNA-181b(miR-181b)在胃癌患者血清中的水平变化及临床意义。方法选取该院2018年3月至2020年9月收治的65例胃癌患者作为胃癌组,选取28例胃癌前病变患者作为癌前病变组,另选取同期32例体检健康者作为健康组。采用实时荧光定量PCR检测血清miR-181b水平,采用酶联免疫吸附试验检测血清癌胚抗原(CEA)水平。比较胃癌组、癌前病变组和健康组血清miR-181b、CEA水平;分析血清miR-181b、CEA水平与胃癌患者临床病理特征的关系;绘制受试者工作特征曲线(ROC曲线)评估血清miR-181b、CEA对胃癌的诊断价值。结果胃癌组血清miR-181b、CEA水平高于健康组和癌前病变组(P<0.05);癌前病变组血清miR-181b、CEA水平高于健康组(P<0.05)。血清miR-181b、CEA水平与胃癌患者TNM分期、有无淋巴结转移、有无远处转移、病理分型、肿瘤最大径、分化程度有关(P<0.05)。Pearson相关分析结果显示,胃癌患者血清miR-181b水平与CEA水平呈正相关(r=0.554,P<0.001)。ROC曲线分析结果显示,血清miR-181b诊断胃癌的曲线下面积为0.873,灵敏度为90.6%,特异度为79.1%,均高于CEA。结论miR-181b在胃癌患者血清中高表达,且与胃癌的发生、发展有关,还可作为胃癌早期诊断的潜在标志物。

Objective To explore the changes and clinical significance of microRNA-181b(miR-181b)in the serum of patients with gastric cancer.Methods A total of 65 patients with gastric cancer admitted to the hospital from March 2018 to September 2020 were selected as the gastric cancer group,28 patients with gastric precancerous lesions were selected as the precancerous lesion group,and 32 patients with healthy physical examination during the same period were selected as the healthy group.Real-time fluorescent quantitative PCR was used to detect serum miR-181b levels,and enzyme-linked immunosorbent assay was used to detect serum carcinoembryonic antigen(CEA)levels.Compared the levels of serum miR-181b and CEA in gastric cancer group,precancerous lesion group and healthy group.Analyzed the relationship between serum miR-181b,CEA levels and clinicopathological characteristics of patients with gastric cancer.The receiver operating characteristic curve(ROC curve)was drawn to evaluate the diagnostic value of serum miR-181b and CEA in gastric cancer.Results The levels of serum miR-181b and CEA in gastric cancer group were higher than those in healthy group and precancerous lesion group(P<0.05).The serum miR-181b and CEA levels in precancerous lesion group were higher than those in healthy group(P<0.05).Serum miR-181b and CEA levels related to TNM staging,with or without lymph node metastasis,with or without distant metastasis,pathological classification,tumor maximum diameter and degree of differentiation in patients with gastric cancer(P<0.05).Pearson correlation analysis showed that serum miR-181b levels in patients with gastric cancer positively correlated with CEA levels(r=0.554,P<0.001).ROC curve analysis results showed that the area under the curve for the diagnosis of gastric cancer by serum miR-181b was 0.873,the sensitivity was 90.6%,and the specificity was 79.1%,which were higher than those of CEA.Conclusion miR-181b highly expresses in the serum of gastric cancer patients and relates to the occurrence and development of gastric cancer.It could also be used as a potential marker for early diagnosis of gastric cancer.