数字化流式形态学尿液分析仪复检规则建立的探讨

Investigation on establishing re-examination rules of digital flow morphological urine analyzer

目的对FUS2000全自动尿液分析仪(以下简称FUS2000)检测尿液有形成分的结果进行分析,建立复检规则。方法随机选取该院2020年3月1日至8月11日收治的接受辅助生殖技术助孕的不孕不育患者的尿液标本682份,同时进行尿液干化学、有形成分分析及人工镜检。以人工镜检结果为“金标准”,计算FUS2000纠正前、后检测红细胞、白细胞、上皮细胞、管型及结晶的真阳性率、真阴性率、假阳性率、假阴性率;比较FUS2000纠正前、后的检测结果;分析不同方法下尿液红细胞及白细胞检测阳性率;建立适合FUS2000的尿液复检规则。结果纠正后FUS2000检测红细胞、白细胞、上皮细胞及结晶的真阳性率较纠正前提高。FUS2000纠正前、后的红细胞、白细胞、上皮细胞、管型及结晶的检测结果比较,差异有统计学意义(P<0.05)。干化学与有形成分分析联合时纠正前红细胞阳性率为18.9%,白细胞阳性率为25.5%,纠正后红细胞阳性率为15.0%,白细胞阳性率为25.4%。结论数字化流式形态学尿液分析仪复检规则的建立应充分考虑仪器对有形成分识别的准确性,以减少复检率,提高工作效率。

Objective To analyze the results of urine visible component detected by the FUS2000 automatic urine analyzer(hereinafter referred to as FUS2000),and establish re-examination rules.Methods Randomly selected 682 urine specimens of infertile patients who received assisted reproductive technology from March 1 to August 11,2020 in the hospital.At the same time,dry chemical,visible component analysis of urine and manual microscopy were performed.Used manual microscopy results as the"gold standard",calculated the true positive rate,true negative rate,false positive rate and false negative rate of red blood cells,white blood cells,epithelial cells,tubular type and crystals before and after correction of FUS2000.Compared FUS2000 detection results before and after correction.Analyzed the positive rate of urine red blood cells and white blood cells under different methods.Established urine re-examination rules suitable for FUS2000.Results The true positive rate of red blood cells,white blood cells,epithelial cells and crystals after correction of FUS2000 was higher than that before correction.There was significant difference on the detection results of red blood cells,white blood cells,epithelial cell,tubular type and crystals before and after correction of FUS2000(P<0.05).In the combined analysis of dry chemistry and visible component,the positive rate of red blood cells before correction was 18.9%,the positive rate of white blood cells before correction was 25.5%,the positive rate of red blood cells after correction was 15.0%,and the positive rate of white blood cells after correction was 25.4%.Conclusion The establishment of re-examination rules for digital flow morphology urine analyzers should fully consider the identification accuracy of the instrument to visible component,so as to reduce the re-examination rate and improve work efficiency.