Anammox的硫酸盐利用特性及脱氮性能

Sulfate Utilization Characteristics and Denitrification Performance of Anammox Process

以厌氧氨氧化(Anammox)污泥为种泥,进水中用SO_(4)^(2-)全部替代NO_(2)^(-)-N作为电子受体来驯化污泥去氧化NH_(4)^(+)-N。反应器运行180 d后,NH_(4)^(+)-N去除量变化情况与Anammox菌活性变化情况相近,系统整体NH_(4)^(+)-N去除量在40 mg/L以上,但SO_(4)^(2-)并未去除,因此认为SO_(4)^(2-)不能作为电子受体来氧化NH_(4)^(+)-N,推测NH_(4)^(+)-N的去除可能存在其他电子受体;向反应器中添加5 mmol/L的羟氨能促进NH_(4)^(+)-N的去除,说明NH_(4)^(+)-N的去除与Anammox菌活性有关。反应器中Candidatus Brocadia和Candidatus Jettenia丰度由2.40%增至15.63%,Nitrosomonas属丰度由0.03%增至6.32%,Nitrobacter属丰度由0.12%增至1.54%,AOB活性大于NOB。菌群数量变化说明反应器中NH_(4)^(+)-N的去除与AOB、NOB以及Anammox活性有关。在批次实验中,向反应瓶中注入微量氧气后,氨氮的去除与AOB、NOB的硝化作用以及Anammox菌活性呈现显著相关,推测反应器中NH_(4)^(+)-N的去除与内部溶解氧量有关。

Anammox sludge was used as the seed sludge,and SO_(4)^(2-)was used to replace NO_(2)^(-)-N as the electron acceptor to domesticate the sludge and oxidize NH_(4)^(+)-N.After the reactor was operated for 180 days,the change of ammonia nitrogen removal in the system was similar to that of Anammox bacteria activity.The overall ammonia nitrogen removal of the system was more than 40 mg/L,but sulfate was not removed.It was concluded that SO_(4)^(2-)could not be used as an electron acceptor to oxidize NH_(4)^(+)-N,and there might be other electron acceptors to oxidize and remove NH_(4)^(+)-N.Adding 5 mmol/L hydroxylamine to the reactor could promote the removal of ammonia nitrogen,indicating that the removal of ammonia nitrogen was related to the activity of Anammox bacteria.The abundance of Candidatus Brocadia and Candidatus Jettenia in the reactor increased from 2.40%to 15.63%;the abundance of Nitrosomonas increased from 0.03%to 6.32%;the abundance of Nitrobacter increased from 0.12%to1.54%,and the activity of AOB was greater than that of NOB.The change in the number of bacteria indicated that the removal of ammonia nitrogen in the reactor was related to the activity of AOB,NOB and Anammox bacteria.In batch experiments,after a trace amount of oxygen was injected into the reaction flask,the removal of ammonia nitrogen was significantly related to the nitrification of AOB and NOB and the activity of Anammox bacteria.It was speculated that the removal of ammonia nitrogen in the reactor was related to the amount of internal dissolved oxygen.