环状RNA Circ-RANBP1在胰腺癌组织中的表达及其对细胞增殖、迁移和侵袭能力的影响

Expression of Circ-RANBP1 in Pancreatic Cancer Tissue and its Effect on Cell Proliferation,Migration and Invasion

背景:大量研究表明,环状RNA(circRNA)异常表达与多种肿瘤的发生、发展、预后等密切相关,是理想的诊断指标和治疗靶点。但circRNA在胰腺癌发生、发展中的作用尚需进一步探究。目的:探讨circ-RANBP1在胰腺癌组织中的表达及其对胰腺癌细胞增殖、迁移和侵袭能力的影响。方法:以原位杂交法检测circ-RANBP1在胰腺癌组织和相应癌旁正常组织中的表达。常规培养MIA PaCa-2细胞和SW 1990细胞,分别转染circ-RANBP1敲低寡聚物和过表达质粒,并设立相应对照组。qRT-PCR法检测circ-RANBP1在胰腺癌细胞中的表达。EdU实验检测circ-RANBP1对细胞增殖能力的影响。Transwell实验检测circ-RANBP1对细胞迁移、侵袭能力的影响。蛋白质印迹法和免疫荧光染色检测circ-RANBP1对细胞上皮-间质转化(EMT)进程的影响。血管形成实验评估circ-RANBP1表达对血管形成能力的影响。结果:Circ-RANBP1在胰腺癌组织中表达增高,且与患者的不良预后密切相关。Circ-RANBP1下调能抑制MIA PaCa-2细胞增殖,而circ-RANBP1过表达可促进SW 1990细胞增殖。与对照组相比,敲低circ-RANBP1能抑制MIA PaCa-2细胞迁移和侵袭,而过表达circ-RANBP1可促进SW 1990细胞迁移和侵袭。敲低circ-RANBP1可抑制EMT,过表达circ-RANBP1可促进EMT。抑制circ-RANBP1表达可明显降低血管形成能力,过表达则可明显促进血管形成。结论:Circ-RANBP1在胰腺癌组织中高表达,并可促进胰腺癌细胞的增殖、迁移、侵袭、EMT和血管形成。

Background:Studies have shown that abnormal expression of circular RNA(circRNA)is closely related to the development,progress and prognosis of a variety of tumors,and is an ideal diagnostic indicator and therapeutic target.However,the role of circRNA in the development and progress of pancreatic cancer needs to be further explored.Aims:To investigate the expression of circ-RANBP1 in pancreatic cancer tissue and its effect on pancreatic cancer cell proliferation,migration and invasion.Methods:The expression of circ-RANBP1 in pancreatic cancer tissue and normal para-cancerous tissue was detected by in situ hybridization.MIA PaCa-2 cells and SW 1990 cells were cultured,and transfected with knockdown oligomer and overexpressed plasmid of circ-RANBP1,respectively,and corresponding control groups were served.Expression of circ-RANBP1 in pancreatic cancer cells was detected by qRT-PCR.EdU method was used to detect the effect of circ-RANBP1 on cell proliferation.Transwell assay was used to detect the effect of circ-RANBP1 on cell migration and invasion.Western blotting and immunofluorescence were used to detect the effect of circ-RANBP1 on epithelial-mesenchymal transition(EMT).Angiogenesis assay was used to explore the effect of circ-RANBP1 on angiogenesis ability.Results:The expression of circ-RANBP1 was significantly increased in pancreatic cancer tissue when compared with paired normal tissues,and was closely associated with poor prognosis of patients.Circ-RANBP1 knockdown inhibited the proliferation of MIA PaCa-2 cells,while overexpression of circ-RANBP1 promoted the proliferation of SW 1990 cells.Compared with control group,circ-RANBP1 knockdown suppressed the migration and invasion of MIA PaCa-2 cells,and overexpression of circ-RANBP1 promoted the migration and invasion of SW 1990 cells.Knockdown of circ-RANBP1 could inhibit EMT,while circ-RANBP1 overexpression showed opposite effect.Inhibition of circ-RANBP1 significantly reduced angiogenesis,while overexpression of circ-RANBP1 significantly enhanced angiogenesis.Conclusions:Circ-RANBP1 is highly expressed in pancreatic cancer tissue,and can promote the proliferation,migration,invasion,EMT and angiogenesis of pancreatic cancer cells.