光质对红阳猕猴桃愈伤组织生长速度和花青素合成的影响

Effects of Light Quality on Callus Growth Rate and Anthocyanin Synthesis of Hongyang Kiwifruit

以富含花青素的绿肉红心猕猴桃红阳为试材,设计6种LED光质,测定不同时期愈伤组织的直径、芽的长度、花青素含量及花青素代谢相关基因的表达,探究不同光质对猕猴桃生长速率以及花青素合成的影响。结果表明,红光对愈伤组织生长的促进作用最为明显,蓝光与红光∶蓝光为3∶1能显著降低愈伤组织和芽的生长速率;而红光下花青素含量降低,花青素合成关键基因CHS、DFR、ANS、UFGT、CRY在红光下的表达受到抑制;蓝光能有效提升愈伤组织花青素的积累和CHS、F3H、DFR、CHI、UFGT、CRY、F3′H等基因的表达水平;红色果肉形成的关键基因MYB110仅在红光∶蓝光为1∶3下高表达。研究结果对猕猴桃及其他果树无性繁殖具有一定参考价值,同时为花青素在不同光质下的调控研究提供新的思路。

Taking the anthocyanin rich green flesh red heart kiwifruit Hongyang as the test material,six different LED light qualities were designed.The diameter of callus,the length of bud,the content of anthocyanin and the expression of genes related to anthocyanin metabolism of Hongyang kiwifruit were measured to explore the effects of different light qualities on the growth rate and anthocyanin synthesis of kiwifruit.The results showed that red light promoted the growth of callus most obviously,while blue light and red light∶blue light=3∶1 treatment could reduce the growth rate of callus and bud.However,the anthocyanin content in kiwifruit tissue decreased under red light,and the expression of key genes CHS,DFR,ANS,UFGT and CRY of anthocyanin synthesis were inhibited under red light.Blue light could effectively enhance the anthocyanin accumulation and the expression levels of CHS,F3H,DFR,CHI,UFGT,CRY and F3′H genes in callus.In addition,MYB110,the key gene of red flesh formation,was only highly expressed in the red light∶blue light=1∶3 treatment.The results not only have potential value for asexual propagation of kiwifruit and other fruit trees,but also can provide new support for the regulation of anthocyanins under different light quality.