应用16S rDNA分析青海喜马拉雅旱獭肠道菌群多样性

Using 16S rDNA to study the diversity of the intestinal flora of the Qinghai Himalayan marmot

目的阐述青海喜马拉雅旱獭肠道菌群组成和群落结构,分析群落分布和种群遗传特征,探讨肠道菌群与环境因素之间的关系。方法对来自青海省3个地理亚区的5个小区共计45份喜马拉雅旱獭粪便样本进行肠道微生物样品的收集,利用16S rDNA技术进行基因测序及微生物多样性分析,得到样本物种分类信息与相对丰度。基于OTU聚类做DCA分析探求肠道菌群与环境因子的关系,使用PICRUSt软件对肠道菌群的功能进行预测。结果喜马拉雅旱獭的肠道菌群主要分布在6个门,10个属,且环境因素中温度、海拔、经度对其影响较大。功能预测发现遗传信息处理与代谢通路在肠道菌群中明显富集。结论通过对青海喜马拉雅旱獭肠道菌群组的研究,为青藏高原的鼠疫防控提供具有实用价值的基础信息,还为及早发现新型病原体和更好的防控动物源性传染病提供理论依据,对未来相应学科的深入研究与快速发展具有重要价值与意义。

Collected intestinal microbial samples from 45 Himalayan marmot feces samples from Qinghai Province,expounded and analyzed the relevant characteristics of the intestinal flora.Using 16S rDNA technology to analyze the sample species classification information and relative abundance.Based on OTU clustering to do DCA analysis to explore the relationship between intestinal flora and environmental factors,and use PICRUSt software to predict the function of intestinal flora.The intestinal flora of the Himalayan marmot is mainly distributed in 6 phyla and 10 genera,and environmental factors have different effects on it.Genetic information processing and metabolic pathways are significantly enriched in the prediction of intestinal flora function.This study provides valuable information for plague prevention and control on the Qinghai-Tibet Plateau,and provides theoretical basis for the discovery of new pathogens and prevention of zoonotic infectious diseases,which is of great value and significance for the development of relevant disciplines in the future.