液相色谱-质谱法测定动物源性食品中的三聚氰酸及其同系物

Detection of cyanuric acid and its homologues in animal products by liquid chromatography mass spectrometry

采用UPLC BEH Amide(2.1 mm×100 mm×1.7μm)色谱柱,以10%乙酸铵水溶液和乙腈为流动相进行梯度洗脱,流速为0.4 mL/min,进样量5μL,柱温为40℃。建立同时检测动物源性食品中三聚氰酸、三聚氰酸一酰胺、三聚氰酸二酰胺含量的高效液相色谱-质谱联用方法。三聚氰酸、三聚氰酸一酰胺、三聚氰酸二酰胺在0.05~250 mg/L浓度范围内线性关系良好(r^(2)≥0.99),加标回收率均在88.0%~102.8%之间。该方法简便、快速、灵敏度高,适用于测定动物源性食品中三聚氰酸、三聚氰酸一酰胺、三聚氰酸二酰胺的含量。

The column adopted UPLC BEH Amide(2.1 mm×100 mm×1.7μm),Gradient elution was performed using 10%ammonium acetate aqueous solution and acetonitrile.The flow rate was set at 0.4 mL/min and injection volume was 5μL,The temperature of chromatographic column was maintained at 40℃.Cyanuric acid,ammelide and ammeline in animal products were determinated by HPLC-MS.The results showed that the concentration range of cyanuric acid,ammelide and ammeline were 0.05~250 mg/L with good linear relationship(r^(2)≥0.99).The recoveries of these three substances were between 88.0%and 102.8%.The method is simple,rapid and highly sensitive,It was suitable for simultaneous determination of cyanuric acid,ammelide and ammeline in animal products.