Effect of rhynchophylline on behavior of zebrafish with Alzheimer disease

OBJECTIVE To investigate the effect of rhynchophylline on behavior of zebrafish with Alzheimer disease induced by AlCl3.METH⁃ODS Take a video of the zebrafish before train⁃ing,which is convenient for judging whether the zebrafish has been trained successfully.Then,the zebrafish were trained for 7 d.The 60 zebraf⁃ish that were successfully trained were randomly divided into 6 groups:normal group,model group,positive group,low-dose rhynchophylline group,middle-dose group,and high-dose group.The normal group was video-recorded,while the model group,positive group,low-dose group,middle-dose group,and high-dose group were given AlCl3 for modeling.After that,the model group was videotaped,and the other groups were given drug intervention.Both the positive group and the rhynchophylline administration group were administered for 6 d,and finally all the administration groups were videotaped.After all the video is finished,the behavioral analysis software smart 3.0 was used for behavioral anal⁃ysis,and conclusions are drawn by analyzing the data.RESULTS The data of the 6 groups of zebrafish staying in the red short arm area were used for comparative analysis:there was a signif⁃icant difference between the normal group and the model group(P<0.05),the model group and the positive group,and the middle-dose group of rhynchophylline There are significant differences in the high-dose group(P<0.05).Comparative analysis with the data of the percentage of zebraf⁃ish in the red short arm area of the 6 groups:the normal group and the model group are signifi⁃cantly different(P<0.05),There were significant differences between the model group and posi⁃tive group,the dose of the rhynchophylline administration group high-dose group and the middle-dose group(P<0.05).Comparing the data of the swimming distance of the 6 groups of zebrafish in the red short arm area:the normal group and the model group are not significantly different(P>0.05),while the model group is only significantly different from the high-dose group(P<0.05).However,the comparison of the percentage of zebrafish swimming distance in the red short arm area of the 6 groups of data:the normal group and the model group are signifi⁃cantly different(P<0.05),the model group and the positive group,and the rhynchophylline administration group There were significant differ⁃ences between the dose group and the high-dose group(P<0.05).CONCLUSION Rhynchophyl⁃line can improve the behavior of zebrafish with Alzheimer disease.