一种相对荧光发射强度标准物质的研制

Development of A Relative Fluorescence Emission Intensity Reference Material

按照国家一级标准物质技术规范的各项要求,以美国国家标准与技术研究院研制的荧光相对发射强度标准物质为参照,采用8家资质实验室进行联合定值,并进行均匀性检验和稳定性考察,研制了4-(二氰基亚甲基)-2-甲基-6-(4-二甲基氨基苯乙烯基)-4H-吡喃(DCM)正丙醇溶液相对荧光发射强度标准物质。结果表明该标准物质具有良好的均匀性,可在4℃下稳定保存12个月,可提供在472 nm激发波长下,波长覆盖范围为490~830 nm、波长间隔为1 nm的标准荧光相对发射强度值。综合考虑样品的均匀性、稳定性检验及定值过程中引入的不确定度,评定了各波长下的不确定度。该标准物质各技术指标符合相关规范的要求,其量值具有准确性和溯源性,已成功获批为国家二级有证标准物质,编号为GBW(E)136758,可用于相关荧光类仪器及检测模块的校准、分析方法评价,以及生物、食品、药品、化工产品及化妆品中的质量控制等方面。

According to the requirements of technical norm of primary reference material,a relative fluorescence emission intensity certified reference material of 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran(DCM)in n-propanol solution was developed,whose references were relative intensity correction standards for fluorescence spectroscopy developed by National Institute of Standards and Technology.A cooperative certification was performed by eight qualified laboratories,and the homogeneity and stability were evaluated.The homogeneity test showed that the standard material had good homogeneity.The stability test showed that it could be stored at 4℃for 12 months.The relative fluorescence emission intensity of DCM in n-propanol solution reference material can provide the standard fluorescence relative emission intensity with wavelength coverage of 490~830 nm and wavelength interval of 1 nm under the excitation of 472 nm.The process of certification,homogeneity and stability test were comprehensively considered to evaluate the uncertainty at each wavelength.The technical indexes of the reference material meet the requirements of relevant specifications,and the values are accurate and traceable.It has been successfully identified as a national gradeⅡcertified reference material,which is numbered GBW(E)136758.It can be used for the calibration of related fluorescent instruments and detection modules,the evaluation of analytical methods,and the quality control of biology,food,medicine,chemical products and cosmetics.