Hg^(2+)探针的合成、光谱性能及生物应用研究

Synthesis, Optical properties and Biological Applications of the Hg^(2+) Probe

设计合成并表征了一种罗丹明类Hg^(2+)探针,对其识别性能进行了研究。检测了探针的紫外吸收光谱及荧光发射光谱,结果表明探针对Hg^(2+)具有较好选择性和灵敏度。探针与Hg^(2+)结合后,观察到吸光度、荧光信号及溶液颜色都发生改变。通过Job′s曲线研究发现Hg^(2+)和探针配合的计量比为1∶1。理论计算表明,探针结构中的S原子和羰基O原子与Hg^(2+)进行配位,诱导内酰胺环打开。将10μmol/L的探针作用于细胞时,存活率约为90%。10μmol/L探针在细胞中几乎无荧光,与Hg^(2+)孵育后可观察到细胞内的荧光明显增强,探针具有很好的细胞膜通透性。在小鼠腹腔内注射探针(400μL×400μmol/L),拍照未发现荧光,在同一位置注射Hg^(2+)后,捕获到明显荧光信号。结果表明,探针具有优良的性能,在生物体染色和标记以及疾病的早期诊断方面都具有潜在的应用价值。

A rhodamine-based probe was developed and characterized.The recognition performance was studied.According to the absorption spectrum and fluorescence emission spectrum,the current probe showed highly selective and sensitive to Hg^(2+).Upon binding with Hg^(2+),comparable amplifications of absorption,fluorescence signals and colours of the solution were observed.Job′s plot indicated a clear 1∶1 stoichiometry of Hg^(2+)and probe.Theoretical calculation revealed that S atom and O atom of carbonyl group coordinate with Hg^(2+),inducing the opening of lactam ring.The cell viability remains about 90%under the treatment of 10μmol/L probe.After cells were incubated with probe(10μmol/L)in culture medium,no fluorescence of probe inside the living cells was observed.After supplemented with 10μmol/L Hg^(2+),a significant increasing of fluorescence intensities from the intracellular area was observed.It was proven that probe was cell-permeable.Fluorescence signal was not detected when the mouse was given an i.p.injection with probe(400μL×400μmol/L).After the injection of Hg^(2+)at the same location,obvious fluorescence signal was noted.The results showed that the probe has excellent performance and potential application value in staining and labelling,and even in early diagnosis of diseases.