补气活血通络方对大鼠创伤性深静脉血栓形成及血红素加氧酶-1基因表达的影响

Efficacy of Buqi Huoxue Tongluo Decoction on Traumatic Deep Vein Thrombosis and Hmox-1 Gene Expression in Rats

目的:观察补气活血通络方对大鼠创伤性深静脉血栓形成及血红素加氧酶-1(Hmox-1)基因表达的影响。方法:采用钳夹大鼠单侧股静脉并予后肢髋人字石膏固定法建立创伤性深静脉血栓模型。将36只SD大鼠随机分为造模组、低浓度中药组、高浓度中药组、阿司匹林组,造模24h后分别使用生理盐水、补气活血通络方或阿司匹林肠溶片对大鼠灌服3d后,检测股静脉血栓干、湿重。运用苏木精-伊红(HE)染色观察股静脉血管形态学改变及血栓情况,实时PCR检测Hmox-1基因的表达情况。结果:造模成功后肉眼可见股静脉血栓形成,HE染色见股静脉管腔内血栓形成。与造模组相比,高浓度中药组和阿司匹林组股静脉中血栓质量明显降低(P<0.05),HE染色镜下也呈现相同趋势;而与造模组相比,低浓度和高浓度中药组中Hmox-1基因呈现浓度依赖性表达增高(P<0.05),但阿司匹林组中Hmox-1基因表达低于两个中药组。结论:补气活血通络方可能通过促进Hmox-1基因表达来降低大鼠创伤性深静脉血栓形成。

Objective:To observe the efficacy of Buqi Huoxue Tongluo decoction on traumatic deep vein thrombosis(DVT)and Hmox-1gene expression in rats.Methods:The rat model of traumatic DVT was established by clamping the unilateral femoral vein and fixing the posterior hip with herringbone plaster.36SD rats were randomly divided into model group,low-concentration Chinese medicine group,high-concentration Chinese medicine group and Aspirin group.24hafter model establishment,the rats were given normal saline,Buqi Huoxue Tongluo decoction or Aspirin enteric-coated tablet for 3d respectively,and the dry and wet weight of femoral vein thrombus were detected.The morphological changes of femoral vein vessels and thrombosis were observed by Hematoxylin-Eosin(HE)staining.The expression of Hmox-1gene was detected by RT-PCR.Results:Femoral vein thrombosis could be seen by naked eyes after the model was successfully established,and intraluminal thrombosis of femoral vein was observed by HE staining.Compared with the model group,the mass of thrombosis in the femoral vein in the high-concentration Chinese medicine group and the Aspirin group was significantly decreased(P<0.05),and the same trend was observed under HE staining microscope.Compared with the model group,the expression of Hmox-1gene in low and high concentration Chinese medicine groups were increased in a concentration-dependent manner(P<0.05),but the expression of Hmox-1gene in Aspirin group was lower than that in the two Chinese medicine groups.Conclusion:Buqi Huoxue Tongluo decoction may reduce traumatic DVT in rats by promoting Hmox-1gene expression.