车前草水提物对环磷酰胺致免疫抑制小鼠免疫功能的影响

The Effects of Plantain aqueous Extract on Immune Functions in Immunosuppressive Mice Induced by Cyclophosphamide

目的:研究车前草水提物(PAE)对CY诱导的免疫抑制小鼠免疫功能的影响,为PAE在临床应用研究提供实验基础。方法:SPF级ICR小鼠60只,随机分为正常对照组、模型组、PAE低、中、高剂量组,每组12只。观察各组小鼠的体重增加率、脏器指数、肠黏膜PPs的数量及淋巴细胞表型、细胞因子水平的改变。结果:注射CY后模型组、PEA低、中剂量组小鼠体重增加率、脏器指数、肠黏膜PPs的数量及细胞因子水平降低(P<0.05),PPs内CD3+细胞比例升高(P<0.05),CD19+细胞比例均降低(P<0.05);尤其以模型组、PEA低剂量组明显(P<0.05)。结论:PAE预处理可改善CY致免疫抑制小鼠的免疫功能。

Objective To study the effects of plantain aqueous extract(PAE)on immune function in immunosuppressive mice inducedcyclophosphamide,and to provide experimental basis for clinical application of PAE.Metheds The 60 SPF ICR mice were randomly divided intocontrol group,model group,low,mediumand highdose groups of PAE,which had 12 mice of each group.The rate of weight gain,viscera index,number of intestinal mucosa PPs,lymphocyte phenotype and cytokine level were observed.Results After the injection of CY,the rate of weight gain,viscera index,the number of PPs and the level of cytokines in the intestinal mucosa of the mice in the model group,low and medium dose groups of PEA were decreased(P<0.05),the proportion of CD3+cells in PPs were increased(P<0.05),while the proportion of CD19+cells were decreased(P<0.05),especially in the model group and the low dose group of PEA(P<0.05).Conclision PAE pretreatment can improve the immune function of CY induced immunosuppressive mice.