小麦种质抗吸浆虫鉴定及抗虫SSR标记开发

OWBM-resistance Screening for Wheat Germplasm and SSR Marker Development in Major Resistant Qsm.hbau-4A

麦红吸浆虫(Sitodiplosis mosellana Géhin)是严重威胁小麦生产的重要害虫,其生活习性隐蔽,防治困难,筛选抗虫小麦种质及可用于抗虫性辅助选择的分子标记意义重大。本研究对我国不同麦区、不同时期的566份小麦种质资源进行了吸浆虫抗性鉴定,并尝试从抗虫性主效QTL所在基因组区段开发新的SSR标记。结果表明,27.03%的供试小麦种质对吸浆虫高抗或免疫,那些早期选育的抗虫品种依然具有很好的吸浆虫抗性。同时,从前期定位的抗麦红吸浆虫主效位点Qsm.hbau-4A所在区段中开发出565个SSR标记,并以11个具有较好多态性的SSR标记,在566份小麦种质资源中对这些标记的检测效率进行了验证,其中标记Xhs87_(201bp)的扩增效果好、检测效率较高(平均65.37%),可以用于抗虫种质资源筛选和分子标记辅助选择。

Orange wheat blossom midge(OWBM,Sitodiplosis mosellana Géhin)is an important pest insect that seriously threatens wheat production.Cryptic habitat of OWBM lifecycle made pest control very difficult.Therefore,screening for OWBM-resistant wheat germplasm and tightly-linked molecular marker is of great important for pest control.In this study,566 wheat germplasm resources from different wheat regions and different periods,and genomic regions surrounding major OWBM locus were used to identify their resistance to OWBM,and to discover new SSR markers,respectively.The results showed that 27.03%of wheat cultivars were immune or highly resistant to OWBM,of which released in early years were still resistant to OWBM.Meanwhile,565 SSR markers were developed from the genomic region of major OWBM-resistant locus(Qsm.hbau-4 A),and their detection efficiency were tested among this panel of wheat accessions.Of these SSR markers,marker Xhs87_(201bp)showed robust PCR amplification and presented an average of 65.37%detection efficiency,and can be used for marker-assisted selection of OWBM resistance.