大豆细胞质雄性不育育性恢复基因Rf3的定位

Mapping of Fertility Restorer Gene Rf3 of Cytoplasmic Male Sterility in Soybean

在植物细胞质雄性不育/恢复系统中,一个不育基因能够被不同的恢复基因所恢复。在利用"三系"法进行杂交大豆选育过程中同样发现,作为父本的恢复系恢复能力不尽相同,可能存在不同的育性恢复基因。本研究以大豆细胞质雄性不育系JLCMS84A为母本,以恢复系JLR1为父本配制杂交组合,以所获得的F2分离群体为试验材料,在大豆盛花期(R2期)根据花粉育性鉴定及显著性分析结果,明确JLR1所含恢复基因为显性单基因配子体遗传模式;结合集群分离分析法(BSA,bulked segregant analysis)和高通量测序技术,将其定位在第9号染色体上;进一步利用简单重复序列(SSR,simple sequence repeat)分子标记将该基因定位在分子标记BARCSOYSSR_09_1151和BARCSOYSSR_09_1183之间,遗传距离分别为0.6cM和0.5 cM,研究结果表明该基因是一个不同于已有研究的新恢复基因位点,将其命名为Rf3。本研究为进一步进行Rf3基因的克隆及鉴定提供了研究基础,同时也为开发与该基因紧密连锁的分子标记,并利用分子标记辅助选择(MAS,markerassisted selection)含有此恢复基因的新恢复系提供了理论依据。

In cytoplasmic male sterility(CMS)/restorer-of-fertility(Rf)system,the male sterility might become fertile once the fertility restorer gene(s)is(are)present.However,different restorer genes may represent levels of restorative ability in the three-line hybrid soybean breeding.In this study,the CMS line JLCMS84 A and its restorer line JLR1 were used as female and male parents,respectively,to construct F2 population used for genetic mapping.Segregation analysis during R2 stage indicated a typical inheritance pattern of gametophytic CMS with fertility restorer being controlled by a single dominant gene.The restorer gene was allocated using BSA-seq on chromosome 9 between SSR markers BARCSOYSSR_09_1151 and BARCSOYSSR_09_1183 with the distances of 0.6 cM and 0.5 cM,respectively.Compared with previous studies,we host a novel restorer gene locus named Rf3.Meanwhile,this study also lay a foundation for further cloning and function identification,as well as marker-assisted selection of new restorer lines which contained this restorer gene.