苦荞转录因子FtNAC11的克隆及其功能分析

Molecular Cloning and Functional Analysis of FtNAC11 from Tartary Buckwheat(Fagopyrum tataricum(L.)Gaertn.)

NAC(NAM、ATAF1/2、CUC2)转录因子在植物响应非生物胁迫中发挥着重要的作用。本研究以苦荞品苦1号为材料,从中克隆得到FtNAC11基因。其cDNA全长为774 bp,编码257个氨基酸,分子量为28954.58 Da,等电点为5.91。氨基酸序列比对和系统进化树分析表明,FtNAC11与拟南芥AtNAC61有较高的同源性。qRT-PCR显示FtNAC11在苦荞中的表达受NaCl和PEG的诱导。转录激活活性分析表明,FtNAC11的C末端具有转录激活活性。亚细胞定位显示,该基因定位于细胞核中。利用根癌农杆菌介导法将FtNAC11基因转入拟南芥,过表达FtNAC11基因显著提高了拟南芥植株的耐盐性和抗旱性。以上结果表明,FtNAC11参与苦荞非生物胁迫适应过程,为进一步研究FtNAC11调控苦荞抗旱和耐盐的分子机制奠定了前期工作基础。

NAC(NAM,ATAF1/2,CUC2)gene plays an important role in responses to plant abiotic stresses.In this study,we cloned FtNAC11 from tartary buckwheat cultivar Pinku No.1.Sequence analysis showed that the full-length coding sequence of FtNAC11 was 774 bp encoding for 257 amino acids residues,with a molecular weight of 28954.58 Da and an isoelectric point of 5.91.The sequence alignment and phylogenetic tree analysis showed that FtNAC11 had higher homology with Arabidopsis thaliana(L.)Heynh.AtNAC61.The expression of FtNAC11 gene was induced under PEG or NaCl treatments.Sub-localization by transient expression and yeast transactivation assay indicated that FtNAC11 encoded for a nuclear transcription activator with an activation domain at the region of C-terminal.Moreover,the coding sequence of FtNAC11 was transformed into Arabidopsis thaliana(L.)Heynh.by Agrobacterium-mediated transformation,and the transgenic plants overexpressing this gene resulted in significant enhancement under drought and salt tolerance treatments.Collectively,these results indicated that FtNAC11 is likely involved in abiotic stress.adaptation,providing a basis for further investigation of its molecular mechanism responding to drought and salt tolerance in tartary buckwheat.