益生菌Probio-X^(®)对远航海员肠道中乳酸菌菌群的影响

Effect of Probiotics Probio-X^(®) on Intestinal Lactic Acid Bacteria in Long-sea-voyage Sailors

为探究远洋航行及益生菌Probio-X^(®)的摄入对肠道乳酸菌的影响,本研究招募27名长远航海员,随机分为两组,分别服用复合益生菌制剂(n=11)和安慰剂(n=16),试验周期为30 d。采用乳酸菌特异性引物扩增与PacBio SMRT测序技术相结合的方式,对长远航海员肠道乳酸菌进行检测。在全部船员肠道中共鉴定到57个属,160个种,主要的优势物种有嗜热链球菌(31.06%)、瘤胃乳杆菌(9.31%)、副血链球菌(6.90%)、唾液链球菌(4.11%)、植物乳杆菌(3.47%)等。在长远航后,益生菌组和安慰剂组海员肠道中乳酸菌特有OTU数量增加,而非度量多维尺度分析(NMDS)结果显示,出海前、后益生菌组和安慰剂组乳酸菌结构并无显著差异,仅在物种水平上部分菌种平均相对含量发生显著变化,如血链球菌和副血链球菌。唾液链球菌和嗜热链球菌是海员出海前肠道中主要的乳酸菌。摄入益生菌后,肠道中链球菌属相对含量较少的部分海员乳酸菌肠型易发生改变,植物乳杆菌和发酵乳杆菌相对含量增加。本研究结果表明,摄入益生菌Probio-X^(®)的长远航海员肠道中副血链球菌和血链球菌显著减少,部分海员的乳酸菌肠型优势物种由链球菌属改变为乳杆菌属,这种改变取决于肠道原始乳酸菌的类别和基础数量。本研究在种水平上分析益生菌Probio-X^(®)对长远航海员肠道乳酸菌多样性的影响,为海员的肠道健康维护提供一个可行的途径。

In order to explore the influence of voyage and the intake of probiotics Probio-X^(®)on the intestinal lactic acid bacteria.Taking 27 long-sea-voyage sailors as the research subjects,they are randomly divided into probiotics group(n=11)and placebo group(n=16).The intestinal LAB was detected by the combination of LAB specific primer amplification and PacBio SMRT sequencing technology A total of 57 genera and 160 species were detected in the intestines of 27 long-sea-voyage volunteers.At the species level,they were mainly composed of Streptococcus thermophilus(31.06%),Lactobacillus ruminis(9.31%),Streptococcus parasanguinis(6.90%),Streptococcus salivarius(4.11%),and Lactobacillus plantarum(3.47%).After the voyage,the number of unique OTUs in the probiotic group and the placebo group increased,and the composition of the intestinal flora changed,however,NMDS analysis showed that there was no significant difference in Lactobacillus structure between the probiotics group and the placebo group,However,the average relative abundance of some species changed significantly,such as Streptococcus sanguinis and Streptococcus parasanguinis.Before the voyage,Streptococcus salivarius and Streptococcus thermophilus are the main LAB in the intestinal tract of seafarers.After ingestion of probiotics,the seafarers intestinal tract with low relative abundance was prone to change,and the relative abandance of Lactobacillus plantarum and Lactobacillus fermentum in the intestinal tract were increased.This study showed that probiotics Probio-X^(®)intervention can significantly inhibit the growth of Streptococcus parasanguinis and Streptococcus sanguinis in the intestinal of seafarers during the long voyage.The intake of probiotics Probio-X^(®)will change the LAB intestinal type of seafarers,but this change depends on the type and basic amount of the original LAB in the intestine.By studying the effect of probiotics Probio-X^(®)on intestinal LAB diversity of seafarers during long voyage,we hope to provide a feasible way to maintain intestinal health of seafarers during long voyage.