青钱柳水提物降血糖及抗发炎功效研究

Studies on Hypoglycemic and Anti-inflammatory Effects of Cyclocarya paliurus Extract

青钱柳是珍贵的药食同源植物。本研究旨在明确青钱柳水提物对链脲佐菌素/烟酰胺(STZ/NA)诱导的糖尿病小鼠的降血糖和抗发炎作用。MTT试验表明,50.0μg/mL青钱柳水提物作用24 h后,胰岛RIN-m5F细胞增殖率达71.0%;血清学分析表明,不同剂量青钱柳水提物灌胃4周后,青钱柳各剂量组可明显降低2型糖尿病小鼠的血糖、血脂和血胆固醇水平,特别是以200.0 mg/mL中剂量组的降血糖和降血脂效果最好,与STZ造模组比较,具有显著性差异(P<0.05);与12-氧-十四烷酰醇-13-乙酸酯(TPA)诱发组相比,25.0 mg/mL青钱柳水提物作用12 h和24 h后,小鼠耳朵的肿胀度分别降低26.9%和27.2%,大于消炎药吲哚美辛(PC组)。研究结果表明,一定剂量的青钱柳水提物通过抑制炎症,促进胰岛细胞增殖,保护胰岛组织,降低血糖和调节脂肪代谢,预防糖尿病及其并发症的发生。本研究结果为青钱柳的精深加工和功能食品开发提供参考。

Cyclocarya paliurus is a medicine food homology plant.This study was designed to investigate the hypoglycemic and anti-inflammatory effects of Cyclocarya paliurus extract.The experimental diabetic mice were induced by streptozotocin/nicotinamide(STZ/NA).According the results of MTT assay,the 50.0μg/mL Cyclocarya paliurus extract(CPE)had the best proliferation rate 71.0%on RIN-m5F cells after treated for 24 h.The serological analysis demonstrated the levels of blood glucose,total cholesterol,triglycerides(TG)were significantly decreased after continuous oral of each dose of CPE for 4 weeks in the mice model of type 2 diabetes.Especially,the 200.0 mg/mL CPE group showed the best effect and significant statistical differences(P<0.05)compared with the STZ group.In addition,compared with the 12-O-tetradecanoylphorbol-13-acetate(TPA)group,the reduction of ear edema was 26.9%and 27.2%respectively after administration of 25.0 mg/mL CPE for 12 and 24 hours in the anti-inflammatory experiment of mice.The inhibition of high dose group was better than the positive control(indomethacin).In conclusion,a certain dose of CPE will help to inhibit inflammation,promote pancreatic cell proliferation,and protect pancreatic tissue,consequently,decrease the level of blood glucose and triglycerides,prevent the developing of diabetes and related complications.The study is helpful for the deep processing and functional food development of Cyclocarya paliurus.