超声微波协同制备粗毛纤孔菌多糖及体外降脂作用的研究

Ultrasonic and Microwave Synergistic Preparation of Polysaccharide from Inonotus hispidus and Its Effect on Lowering Lipid in Vitro

本文对粗毛纤孔菌多糖的提取及体外降脂作用进行研究,为粗毛纤孔菌多糖的开发利用提供理论依据。试验以粗毛纤孔菌为原料,以多糖提取率为考察指标,采用单因素实验和Box-Behnken试验设计研究了超声微波协同制备粗毛纤孔菌多糖(IHP)的工艺,对比分析了热水提取法和超声波辅助法对IHP提取率和体外胆酸盐结合能力的影响。结果表明,IHP的最佳提取工艺参数为:料水比1:33 g:mL,微波时间50 s,微波功率500 W,超声时间51 min,超声波功率200 W,此条件下多糖提取率为85.61%。与超声波辅助法和热水提取法相比,提取率分别增加了24.87%和36.38%。三种提取方法制备的IHP体外胆酸盐结合实验结果表明,IHP对牛磺胆酸钠和甘氨胆酸钠具有显著的结合能力,与多糖剂量呈正相关,且IHP对牛黄胆酸钠的结合能力强于甘氨胆酸钠。IHP在相同质量浓度条件下,三种提取方法制备的多糖对胆酸盐的结合能力为超声微波辅助提取>超声辅助提取>热水浸提,且超声微波辅助制备的多糖对甘氨胆酸钠和牛磺胆酸钠结合率分别为30.93%、32.13%。本研究表明超声微波辅助提取法能够显著提高IHP提取效果和体外结合胆酸盐的能力,为制备高活性IHP及其开发利用提供理论依据。

In this study,Inonotus hispidus was used as a raw material,extraction rate of polysaccharide was used as the index,the single factor experiments and Box Behnken experiment design were used to study the preparation process of Inonotus hispidus polysaccharide(IHP)by ultrasound and microwave synergistic extraction,and the effects of several extraction methods on the extraction percentage of IHP and the bile acid binding capacity in vitro were compared and analyzed.The results showed that,the optimal process parameters for the extraction of IHP were:Material-to-water ratio 1:33 g:mL,microwave time 50 s,microwave power 500 W,ultrasonic time 51 min,ultrasonic power 200 W,the extraction percentage of polysaccharide under this condition was 85.61%.Compared with ultrasonic assisted extraction and hot water extraction,the extraction rate increased by 24.87%and 36.38%,respectively.The IHP had significant binding ability to sodium taurocholate and sodium glycocholate,which was positively correlated with the dosage of polysaccharide,and the binding ability of IHP to sodium taurocholate was higher than sodium glycocholate.And under the same mass concentration conditions,the binding ability of polysaccharides prepared by the three extraction methods to bile salts was ultrasonic and microwave synergistic extraction>ultrasonic assisted extraction>hot water extraction,and the binding rates for sodium glycocholate and sodium taurocholate were 30.93%and 32.13%,respectively.This study showed that the ultrasonic and microwave synergistic extraction method could significantly improve the extraction effect of IHP and the ability to bind cholate in vitro,and would provide a theoretical basis for the preparation and development and utilization of highly active IHP.