桦褐孔菌与其固体发酵物对2型糖尿病大鼠的氧化应激损伤作用

Effects of Sclerotium and Solid Fermentation Products of Inonotus obliquus on Inflammation and Oxidative Stress in Diabetic Rats

目的:研究比较桦褐孔菌菌核、固体发酵物和二者的水提物给药对糖尿病大鼠机体炎症和氧化应激损伤的作用。方法:本研究采用高糖高脂饮食联合STZ诱导的2型糖尿病大鼠模型,监测大鼠体重、口服糖耐量试验、酶联免疫吸附试验(ELISA)检测大鼠血清中炎症因子白细胞介素IL-6、IL-1β,肿瘤坏死因子TNF-α、超氧化物歧化酶SOD及丙二醛MDA的含量。结果:与模型组相比,桦褐孔菌IO 3.08、6.16 g/kg剂量组、桦褐孔菌水提物WIO 0.4 g/kg剂量组、桦褐孔菌固体发酵物水提物WSFIO 0.8 g/kg剂量组均可显著提高糖尿病大鼠体重;固体发酵物SFIO 1.3、2.6 g/kg剂量组能显著地改善大鼠口服糖耐量(P<0.05,P<0.01);WIO 0.4、0.8 g/kg剂量组、WSFIO 0.4 g/kg剂量组均可显著降低血清中IL-1β的水平;IO 3.08、6.16 g/kg剂量组、WIO 0.4 g/kg剂量组、SFIO 1.3、2.6 g/kg剂量组和WSFIO 0.8 g/kg剂量组均可显著降低血清中TNF-α的水平;桦褐孔菌各给药组在不同程度上均可显著提高血清中SOD的水平(P<0.05,P<0.01或P<0.001);桦褐孔菌各给药组均可降低血清中MDA的水平(P<0.01或P<0.001)。结论:桦褐孔菌固体发酵物具有良好的抗炎及抗氧化活性。直接以菌粉、固体发酵物或二者的水提物给药对各个指标的影响不一,总体来说,固体发酵物代替野生桦褐孔菌改善2型糖尿病的炎症及氧化应激具有可行性。

Objective:To evaluate the effects of sclerotium and solid fermentation products of Inonotus obliquus on inflammation and oxidative stress in diabetic rats.Methods:The type 2 diabetic rat model were induced by high-glucose-fat diet together with STZ in this study,body weight,oral glucose tolerance test was performed,serum IL-6,IL-1β,TNF-α,SOD and MDA levels were measured using the enzyme-linked immunosorbent assay(ELISA).Results:Compared with the model group,body weight was significantly improved by I.obliquus group(IO 3.08 g/kg,IO 6.16 g/kg),the water extract of I.obliquus group(WIO 0.4 g/kg),the water extract of solid fermentation products of I.obliquus group(WSFIO 0.8 g/kg).Glucose tolerance were significantly improved by solid fermentation products of I.obliquus group(SFIO 1.3 g/kg,SFIO 2.6 g/kg).The IL-1βlevel were significantly reduced by WIO(0.4,0.8 g/kg)and WSFIO(0.4 g/kg).The TNF-αlevel were significantly reduced by IO(3.08,6.16 g/kg),WIO(0.4 g/kg),SFIO(1.3,2.6 g/kg)and WSFIO(0.8 g/kg).The level of SOD and MDA were significantly advanced and decreased respectively by all the medicated group.Conclusion:Solid fermentation products of I.obliquus showed antioxidative and anti-inflammatory effects in diabetic rats.The direct administration of I.obliquus powder,solid fermentation products or water extract of both two had different effects on various indicators.In general,it was feasible to replace solid fermentation products with feral I.obliquus to ameliorate inflammation and oxidative stress in type 2 diabetes.