沙棘粕槲皮素体外抗氧化及对衰老模型小鼠保护作用的研究

Study on Antioxidant Activity of Quercetin from Seabuckthorn Meal in Vitro and Its Protective Effect on Aging Mice

以沙棘粕槲皮素为研究对象,探索其体外抗氧化活性和对衰老模型小鼠的保护作用。采用1,1-二苯基-2-三硝基苯肼(DPPH·)法、超氧阴离子(O_(2)^(-)·)法、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS^(+)·)法、高铁离子还原(FRAP)法,四种方法对沙棘果槲皮素体外抗氧化活性进行研究。通过颈部皮下注射D-半乳糖建立小鼠衰老模型,用不同剂量的(100、250、500 mg/kg)沙棘粕槲皮素灌胃处理小鼠,眼球取血测血清中总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;取心脏、肾脏和肝脏做苏木精-伊红染色(HE)病理学切片,观察其组织细胞病理状态。结果表明:沙棘粕槲皮素对DPPH·、O_(2)^(-)·、ABTS^(+)·具有良好的清除能力。与模型组比较,沙棘粕槲皮素低剂量组的MDA含量显著降低(P<0.05),中、高剂量组小鼠血清中T-AOC、GSH-PX、SOD活力显著升高(P<0.05),MDA含量显著降低(P<0.05)。从病理组织切片看,随着沙棘粕槲皮素剂量的增加,心肌纤维排列有序,横纹清晰;肾小囊和肾小球形态逐渐正常,肾小管数量增多且排列紧密;肝细胞数量增多,细胞形态完整,窦间隙逐渐显著。由此可知,沙棘粕槲皮素能提高衰老模型小鼠抗氧化酶活性和还原性物质含量,降低脂质过氧化物含量,从而提高衰老模型小鼠的抗氧化能力,对衰老模型小鼠有较好的保护作用。

The antioxidant activity and protective effect of quercetin from seabuckthorn meal were studied in vitro.The antioxidant activities of quercetin in seabuckthorn were studied by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH·)superoxide anion(O_(2)^(-)·),2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt(ABTS^(+)·)and high iron ion reduction(FRAP).The aging model of mice was established by subcutaneous injection of D-galactose into the neck.The mice were treated with quercetin of seabuckthorn meal at different doses(100,250,500 mg/kg)by gavage.The eyeball blood was taken to measure the activities of total antioxidant capacity(T-AOC),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)and malondialdehyde(MDA)in serum;the heart,kidney and liver were taken as hematoxylin eosin staining(HE)to observe the pathological state of the cells.The results showed that quercetin from seabuckthorn meal had good scavenging ability on DPPH·,O_(2)^(-)·and ABTS^(+)·.Compared with the model group,the content of MDA in the low dose group of quercetin of seabuckthorn meal decreased significantly(P<0.05),the activities of T-AOC,GSH-Px and SOD in the serum of mice in the medium and high dose groups increased significantly(P<0.05),and the content of MDA decreased significantly(P<0.05).From the pathological section,with the increase of quercetin dosage in seabuckthorn meal,the myocardial fibers arranged orderly and the transverse lines were clear;the morphology of renal sacs and glomeruli gradually became normal,the number of renal tubules increased and arranged closely;the number of hepatocytes increased,the cell morphology was complete,and the sinus space gradually became significant.Therefore,quercetin from seabuckthorn meal could improve the activity of antioxidant enzymes and the content of reducing substances in aging model mice,reduce the content of lipid peroxide,so as to improve the antioxidant capacity of aging model mice,and had a better protective effect on aging model mice.