2’-岩藻基乳糖对益生菌定殖及抗炎能力的影响

Effects of 2’-Fucosyllactose on the Colonization and Anti-inflammatory Property of Probiotics

目的:探究2’-岩藻基乳糖(2’-Fucosyllactose,2’-FL)对乳酸菌和双歧杆菌增殖、粘附肠道细胞以及抗炎作用的影响。方法:以2’-FL和实验室的30株乳酸菌和双歧杆菌为研究对象,通过测定生物量、产酸和细胞粘附倍数的变化筛选出2’-FL可以增强定殖能力的菌株,再利用脂多糖诱导的RAW264.7细胞炎症模型进一步筛选出其中的潜在抗炎菌株,最后探究2’-FL和潜在抗炎菌株的联合抗炎效果。结果:30株实验菌株中,2’-FL仅能促进两歧双歧杆菌FL-276.1和FL-228.1的增殖,提高乳酸菌ML-1、FN515、FN518、FN249、ML329和双歧杆菌FL-276.1粘附Caco-2细胞的能力。其中两歧双歧杆菌FL-276.1、FL-228.1和鼠李糖乳杆菌FN518可以显著(P<0.05)降低脂多糖诱导的Raw264.7细胞炎症因子NO、TNF-α、IL-6和IL-1β的分泌。2’-FL可以显著降低NO、IL-6和IL-1β的分泌。2’-FL与上述3株菌联用具有协同抗炎作用,但协同效果具有菌株差异性,其中FL-276.1与2’-FL协同抗炎效果最好。结论:2’-FL可以提高乳酸菌和双歧杆菌的定殖能力,并协同发挥抗炎功能,但效果具有菌株差异性,这一结果可以为预防早产儿坏死性小肠结肠炎提供新的选择。

Purpose:This study aimed to investigate the effect of 2’-fucosyllactose(2’-FL)on the proliferation,adhesion to intestinal cells and anti-inflammatory ability of lactic acid bacteria and bifidobacterial.Methods:2’-FL was used to study if the strains could utilize it for promoting proliferation or improving adhesion,by determining the biomass,acid production and variations in adhesion capacity.Then finding out the potential anti-inflammatory strains among the strains whose colonization ability was enhanced by 2’-FL,by a lipopolysaccharide(LPS)-induced macrophage RAW264.7 inflammatory model.Finally,2’-FL and the combinations of 2’-FL and the potential anti-inflammatory strains were used to investigate their effects on the inflammation.Results:Among the thirty experimental strains,2’-FL could promote the proliferation of Bifidobacterium bifidum(FL-276.1,FL-228.1)and improve the adhesion of lactic acid bacteria(ML-1,FN515,FN518,FN249,ML329)and bifidobacterial(FL-276.1)to Caco-2 cells.Bifidobacterium bifidum FL-276.1,Bifidobacterium bifidum FL-228.1 and Lactobacillus rhamnosus FN518 could significantly reduce the secretion of NO,TNF-α,IL-6 and IL-1βin the inflammatory model.Meanwhile,2’-FL alone significantly reduced the secretion of NO,IL-6 and IL-1β.The combination of 2’-FL and the above three strains had a synergistic anti-inflammatory effect,which showed a strain-specific trait.Particularly,strain FL-276.1 combined with 2’-FL exerted the best anti-inflammatory effect.Conclusions:2’-FL could promote the colonization and anti-inflammatory activity of certain lactic acid bacteria and bifidobacterial,which showed a strain-specific trait.It might provide alternative choice for the prevention of necrotizing enterocolitis(NEC)in preterm infants.