Plackett-Burnman联合响应面法优化黑果枸杞黄酮提取工艺及抗氧化性研究

Optimization of Extraction Process of Flavonoids from Lycium ruthenicum Murr.by Plackett-Burnman with Response Surface Methodology and Its Antioxidation Activity

Plackett-Burnman(PB)联合响应面法优化黑果枸杞黄酮提取工艺并评价其抗氧化性。依次通过PB实验设计、最陡爬坡实验设计研究了5种影响因素对提取率的影响,确定了显著性影响因素及响应面中心,采用Box-Behnken(BB)实验设计优化了提取工艺;采用FRAP法及ABTS、DPPH法评价了黑果枸杞黄酮的抗氧化性,并与抗坏血酸进行了平行比较。结果表明,最佳提取工艺参数为:51%乙醇(体积分数)与原料之比为30:1(mL/g),62℃、240 W超声辅助提取42 min,提取率可达42.0974 mg/g(原料),与理论预测值的相对标准偏差为3.24%;FRAP法及ABTS、DPPH法均表明,黑果枸杞总黄酮还原力强于抗坏血酸,其对ABTS、DPPH自由基清除的IC_(50)分别为0.0252、0.0272 mg/mL,均较抗坏血酸强,且二者抗氧化性均与浓度呈量效关系,为开发黑果枸杞资源、拉长其产业链提供了一定的理论基础。

To optimize the extraction conditions of flavonoids from Lycium ruthenicum by Plackett-Burnman combined with response surface methodology and evaluate its antioxidant activity.The factors with significant influence and response surface center were determined by investigating the effects of five factors on extraction rate based on Plackett-Burnman design test and the steepest ascent serach experiment.Meanwhile,the extraction technology was optimized by Box-Behnken response surface methodology.The antioxidant activity of flavonoids from Lycium ruthenicum Murr.was evaluated by using FRAP,ABTS and DPPH,which was then compared with that of ascorbic acid in parallel.Results indicated that the optimized process conditions were as follows:Ethanol volume fraction 51%,liquid to solid ratio 30:1,extraction temperature 62℃,extraction time 42 min,ultrasonic power 240 W,the extraction rate was up to 42.0974 mg/g(raw material)and the RSD with the theoretical value was 3.24%.The antioxidant activity tests revealed that the ferric reducing capacity of flavonoids from Lycium ruthenicum Murr.was stronger than that of ascorbic acid,the IC_(50)of ABTS and DPPH radical scavenging activities of Lycium ruthenicum Murr.flavonoids were 0.0252 and 0.0272 mg/mL,respectively,which were higher than those of ascorbic acid,and the antioxidant activity of flavonoids followed a dosedependent manner at all the tested concentrations.This study would provide a theoretical basis for the further development of Lycium ruthenicum Murr.resources and the extension its industrial production chain.