不同光配比对福鼎大白茶叶片生理和主要氨基酸积累的影响

Effect of Different Light Ratio on Physiology and Main Amino acid Accumulation of Fuding-dabaicha Leaf

研究不同光配比对一年生福鼎大白茶树幼苗叶片生理及主要氨基酸含量的影响。在LED光谱可调人工气候箱内,设置高红光占比光源L1(红光占比71.8%)、高蓝光占比光源L2(蓝光占比62.7%)、红蓝混合光源L3(红光占比43.1%、蓝光占比29%、绿光占比27.9%)和对照白光L0(绿光占比82.1%)四种光处理,在100μmol·m^(−2)·s^(−1)光子通量密度、12 h光周期条件下实施试验,采用氮平衡指数仪测定叶片生长指数,氨基酸自动分析仪测定茶氨酸、谷氨酸和天冬氨酸含量。结果表明,随着处理时间的增加,各光处理均显著增加了茶树叶片叶绿素指数和氮平衡指数,与L0相比,L1处理显著提高茶树叶片氮平衡指数(P<0.05),其次为L3处理,类黄酮指数及茶多酚含量各光处理间无显著差异(P>0.05)。与L0相比,L3和L2处理下叶片茶氨酸、谷氨酸、天冬氨酸含量显著提高(P<0.05)。综上,L3处理(红光、蓝光、绿光配比均衡)更有利于茶树叶片生长及氨基酸积累,能兼顾茶叶产量与品质,是适宜茶树栽培的LED光源。

To explore the effect of different ratio of light on physiological reaction and main amino acid accumulation of annual Fuding-dabaicha leaves.The L1(red light accounted for 71.8%),L2(blue light accounted for 62.7%),L3(red 43.1%,blue 29%,green accounted for 27.9%)and L0(green light accounted for 82.1%)were set in artificial climate boxes with 100μmol·m^(−2)·s^(−1) photosynthetic photon flux density and 12 h photo period.The leaf growth index were determined by nitrogen balance index instrument and the theanin,glutamate and aspartate contents were determined by an automatic amino acid analyzer.The results showed that with the increase of treatment time,the chlorophyll index and nitrogen balance index of tea leaves were significantly increased by all light treatments.Compared with L0,L1 significantly increased the nitrogen balance index of tea leaves(P<0.05),followed by L3,index of flavonoids and polyphenols content between different light treatment had no significant difference.Compared with L0,the content of theanine,glutamate and aspartate under L3 and L2 treatments increased significantly.In conclusion,L3(an equilibrium ratio of red,blue and green light)can be considered as a suitable artificalight source for tea leaves growth and to ensure the maximun retention of main amino acid.