不同蕨菜制品醇提物体外抗氧化及降血糖活性研究

Antioxidant and Hypoglycemic Activities of Ethanol Extracts from Different Products of Blechnum orientale L.in Vitro

目的:比较研究不同蕨菜制品醇提取物的体外抗氧化和降血糖活性。方法:分别采用Folin-Ciocalteu法、NaNO_(2)-Al(NO_(3))3法测定总酚和总黄酮含量;通过DPPH法、ABTS法、普鲁士蓝法、α-葡萄糖苷酶和α-淀粉酶的抑制活性评价体外抗氧化、降血糖活性;Pearson法分析成分含量与活性的相关性。结果:不同蕨菜醇提物的总酚和总黄酮含量、抗氧化活性、α-葡萄糖苷酶和α-淀粉酶抑制活性差异显著。其中鲜品醇提取物的总酚、总黄酮含量最高,分别可达(593±3.45)mg GA/g、(156.75±1.28)mg RT/g,且鲜品醇提取物铁离子还原能力、ABTS自由基清除能力、DPPH自由基清除能力最强;湿品醇提取物对α-葡萄糖苷酶、α-淀粉酶的抑制作用最强。不同蕨菜醇提取物总酚、总黄酮的含量与其抗氧化活性均呈极显著正相关关系(P<0.01),与降血糖活性均呈显著正相关关系(P<0.05)。结论:蕨菜鲜品抗氧化活性较强,湿品降血糖活性较强,可能活性成分为多酚类和黄酮类成分。

Objective:The antioxidant and hypoglycemic effects in vitro of the extract from Blechnum orientale L.were compared.Methods:By using Folin-Ciocalteu method,the total polyphenol content of extracts were determined.The content of flavonoids was determined by NaNO_(2)-Al(NO_(3))3 method.In addition,radicals scavenging(DPPH·,ABTS+·),reducing ability,α-glucosidase andα-amylase inhibitory were carry out to evaluate their antioxidant and hypoglycemic activities.Pearson correlation analysis method was used to measure the correlation of component content and activities.Results:The results showed that there were significant differences in the content of total phenolic and flavonoids,antioxidant activity andα-glucosidase inhibitory activity among different processing products of ethanol extracts from Blechnum orientale L.The alcohol extract from fresh product had the highest content of total phenols((593±3.45)mg GA/g)and total flavonoids((156.75±1.28)mg RT/g),and the fresh product showed the strongest iron ion reduction ability,ABTS free radical scavenging ability and DPPH free radical scavenging ability.The alcohol extract from wet product had the strongest inhibitory effect onα-glucosidase andα-amylase.The contents of total phenols and flavonoids in Blechnum orientale L.with different processing methods were significantly positively correlated with their antioxidant(P<0.01)and were significant positive correlated with their hypoglycemic activity(P<0.05).Conclusion:The alcohol extract from fresh product had the highest antioxidant activity and wet product had the strongest hypoglycemic activity in vitro.The active ingredient may be total phenolic and flavonoids.