鸡桑、华桑和桑的根皮中7种核苷类成分的含量测定

Determination of 7 Nucleosides Contents in Cortex of Morus australis,Morus cathayana and Morus alba

目的:采用高效液相色谱法(HPLC)对不同产地鸡桑、华桑和桑的根皮中腺嘌呤、腺苷、胞苷、次黄嘌呤、鸟苷、尿嘧啶和2'-脱氧腺苷等7种核苷进行测定与分析,为制订质量标准提供参考。方法:采用Venusil MP C18(5μm,250 mm×4.6 mm)色谱柱,以甲醇和水为流动相,梯度洗脱,检测波长为260 nm,流速为1.0 mL/min,柱温30℃。结果:7种核苷成分在各自质量浓度范围内线性关系良好(r≥0.9998),平均回收率为97.49%~101.45%,RSD≤3%。不同产地鸡桑、华桑和桑的根皮中7种核苷的含量差异较大,其中华桑与鸡桑的根皮中尿嘧啶和次黄嘌呤含量整体较高,而不同样品次黄嘌呤含量差异性较大,胞苷含量最低。桑的根皮中鸟苷和腺嘌呤含量较高,2'-脱氧腺苷含量较低。鸡桑与华桑中核苷成分略低于桑。独立样本t检验结果表明,鸡桑、华桑和桑的根皮中7种核苷类成分无显著性差异(P>0.05),其核苷类成分具有一定的等同性。主成分分析和聚类分析结果表明,鸡桑、华桑和桑不能依据核苷类成分含量的差异加以区分,表明以华桑和鸡桑来源的崖桑皮可作为桑白皮的代替使用。结论:鸡桑、华桑和桑的根皮中核苷类成分含量无明显差异,本研究为鸡桑、华桑和桑质量等同性的研究提供了科学依据。

Objective:Seven nucleosides,including adenine,adenosine,cytidine,hypoxanthine,guanosine,uracil and 2'-deoxyadenosine,were determined and analyzed by HPLC in the root bark of Morus australis,Morus cathayana and Morus alba from different areas,so as to provide reference for formulating quality standards.Methods:A venusil MP C18(5μm,250 mm×4.6 mm)column was used.The mobile phases of methanol and water were used for gradient elution.Detection wavelength was 260 nm.The flow rate was 1.0 mL/min.The column temperature was 30℃.Results:There was a good linear relationship between the seven nucleosides in their respective concentration ranges(r≥0.9998).The average recoveries were 97.49%~101.45%with RSD≤3%.There were significant differences in the contents of seven nucleosides in the root bark of Morus australis,Morus cathayana and Morus alba from different producing areas.The contents of uracil and hypoxanthine in the root bark of Morus alba and Morus australis were higher,but the contents of hypoxanthine in different varieties were different,and the content of cytidine was the lowest.The content of guanosine and adenine in root bark of Morus alba was higher than that of 2'-deoxyadenosine.The nucleoside composition of Morus australis and Morus cathayana was slightly lower than that of Morus alba.The results of independent sample t-test showed that there was no significant difference among the seven nucleosides in the root bark of Morus australis,Morus cathayana and Morus alba(P>0.05)and its nucleoside components had certain isotropy.The results of principal component analysis and cluster analysis showed that Morus australis,Morus cathayana and Morus alba could not be distinguished according to the difference of nucleoside content,indicating that Australis Cortex Mori from Morus australis,Morus cathayana could be used as the substitute of Cortex Mori.Conclusion:There is no significant difference in nucleoside contents among the root bark of Morus australis,Morus cathayana and Morus alba.This study provides a scientific basis for the study on the quality equivalence of Morus australis,Morus cathayana and Morus alba.