血清miR-15b、DCLK1水平与宫颈癌患者临床病理特征及预后的关系

Relationships between serum miR-15b,DCLK1 levels and clinicopathological features and prognosis of patients with cervical cancer

目的探讨宫颈癌患者血清miR-15b、双皮质素样激酶1(DCLK1)水平与临床病理特征及预后的关系。方法选取155例宫颈癌患者(研究组),同时选取150例健康者作为对照组。采用实时荧光定量PCR技术测定宫颈癌患者血清miR-15b,采用酶联免疫吸附法(ELISA)检测血清DCLK1,并分析两指标与宫颈癌患者临床病理特征和预后的关系。结果研究组血清miR-15b水平较对照组降低,而血清DCLK1水平较对照组升高(P均<0.05)。血清miR-15b、DCLK1水平均与宫颈癌患者FIGO分期和淋巴结转移有关(P均<0.05)。血清miR-15b低水平者3年总生存率较高水平者降低,而DCLK1高水平者3年总生存率较低水平者降低(P均<0.05)。Cox回归分析显示,FIGO分期ⅢA+ⅢB期、低水平miR-15b及高水平DCLK1是影响宫颈癌患者预后的独立危险因素(P均<0.05)。结论宫颈癌患者血清DCLK1水平升高,而血清miR-15b水平降低,且二者均与FIGO分期、淋巴结转移有关,对评估预后具有重要的临床价值。

Objective To explore the relationships between the levels of serum miR-15b and doublecortin-like kinase 1(DCLK1)and the clinicopathological features and prognosis of patients with cervical cancer.Methods Totally 155 patients with cervical cancer(study group)and 150 healthy people(control group)were selected.The expression of serum miR-15b of patients with cervical cancer was measured by fluorescence real-time quantitative PCR(qRT-PCR),and the level of serum DCLK1 was detected by enzyme-linked immunosorbent assay(ELISA).The relationships between the two indexes and clinicopathological features and prognosis were analyzed.Results The expression of serum miR-15b of the study group was lower than that of the control group,and the level of serum DCLK1 was higher than that of the control group(both P<0.05).Serum miR-15b and DCLK1 were related to FIGO stage and lymph node metastasis in patients with cervical cancer(all P<0.05).The 3-year overall survival rate of patients with low miR-15b expression was lower than that of patients with high miR-15b expression,while the 3-year overall survival rate of the high DCLK1 expression group was lower than that of the low DCLK1 expression group(all P<0.05).Cox regression analysis showed that FIGO stageⅢA-ⅢB,low expression of serum miR-15b and high expression of DCLK1 were independent risk factors for prognosis of cervical cancer patients(all P<0.05).Conclusion The level of serum DCLK1 increases and the level of serum miR-15b decreases in patients with cervical cancer.Both of them are related to FIGO stage and lymph node metastasis.They have important clinical value in evaluating the prognosis.