NVG患者血清及房水中ET-1、MMP、TIMP-2水平变化及其临床意义

Changes of ET-1,MMP and TIMP-2 levels in serum and aqueous humor of NVG patients and their clinical significance

目的观察新生血管性青光眼(NVG)患者血清及房水中血清内皮素(ET-1)、基质金属蛋白酶(MMP)、基质金属蛋白酶抑制剂-2(TIMP-2)的水平变化及其临床意义。方法选取2019年6月至2020年12月枣庄市中医医院收治的45例(45眼)NVG患者作为观察组,另选取同期48例(48眼)年龄相关性白内障(ARC)患者作为对照组,比较两组患者血清及房水中ET-1、MMP、TIMP-2水平变化情况,按照患者发病情况将NVG患者分为急性NVG组(24眼)与慢性NVG组(21眼),比较急慢性NVG患者血清及房水中ET-1、MMP、TIMP-2水平变化情况,并观察ET-1、MMP、TIMP-2水平与NVG的关系。结果观察组患者房水ET-1、MMP、TIMP-2水平均高于对照组,差异有统计学意义(P<0.05)。两组患者血清ET-1、MMP、TIMP-2水平比较差异无统计学意义(P>0.05)。急性NVG患者房水中ET-1、MMP、TIMP-2水平高于慢性NVG患者,差异有统计学意义(P<0.05)。急性NVG患者血清中ET-1、MMP、TIMP-2水平低于慢性NVG患者,但差异无统计学意义(P>0.05)。ROC曲线分析显示,房水ET-1、MMP、TIMP-2用于评估NVG患者诊断价值的AUC分别为0.816、0.981、0.962,评估急慢性NVG的AUC分别为0.654、0.857、0.760。结论房水ET-1、MMP、TIMP-2水平与NVG发生密切相关,检测房水ET-1、MMP、TIMP-2水平可为NVG发病情况提供参考。

Objective To observe the changes of endothelin(ET-1),matrix metalloproteinases(MMP),matrix metalloproteinase inhibitor-2(TIMP-2) levels in serum and aqueous humor of patients with neovascular glaucoma(NVG) and their clinical significance.Methods 45 patients(45 eyes) with NVG treated in Zaozhuang Hospital of Traditional Chinese Medicine between June 2019 and December 2020 were selected as the observation group,and 48 patients(48 eyes) with age related cataract(ARC) during the same period were selected as the control group.The levels of ET-1,MMP and TIMP-2 in serum and aqueous humor were compared between the two groups.Patients with NVG were divided into the acute NVG group(24 eyes)and the chronic NVG group(21 eyes) according to the incidence of patients.The changes of serum and aqueous humor levels of ET-1,MMP and TIMP-2 were compared between patients with acute and chronic NVG,and the relationship between the levels of ET-1,MMP and TIMP-2 and NVG was observed.Results The levels of aqueous humor ET-1,MMP and TIMP-2 in the observation group were higher than those in the control group(P<0.05).There were no statistical differences between the two groups of patients in terms of serum ET-1,MMP and TIMP-2 levels(P>0.05).The levels of ET-1,MMP and TIMP-2 in the aqueous humor of patients with acute NVG were higher than those of patients with chronic NVG(P<0.05).The levels of ET-1,MMP and TIMP-2 in serum of patients with acute NVG were lower than those of patients with chronic NVG(P>0.05).ROC curve analysis showed that the AUC values of aqueous humor ET-1,MMP and TIMP-2 were0.816,0.981 and 0.962 in assessing the diagnostic value of patients with NVG,and were 0.654,0.857 and 0.760 in evaluating acute and chronic NVG.Conclusion The levels of ET-1,MMP and TIMP-2 in aqueous humor are closely related to the occurrence of NVG.The detection of levels of ET-1,MMP and TIMP-2 in aqueous humor can provide references for the occurrence of NVG.