miR-34a蛋白在子宫内膜癌细胞中的表达及其对癌细胞增殖凋亡机制的影响

The expression of miR-34a protein in endometrial cancer cells and its effect on the mechanism of cancer cell proliferation and apoptosis

目的探讨miR-34a在子宫内膜癌细胞组织中的表达及其对子宫内膜癌细胞增殖和凋亡的影响。方法经荧光定量PCR法检测miR-34a在子宫内膜癌细胞组织、癌旁组织内表达,并经双荧光素酶报告基因测定miR-34a靶基因,选择子宫内膜癌细胞Ishikawa开展miR-34a mimics、对照miR-NC转染,并对Notch 1表达载体进行转染;经MTT法、流式细胞术对miR-34a.Notch 1干扰子宫内膜癌miR-34a细胞增殖、凋亡进行分析,Western-blot法对miR-34a干扰Notch 1蛋白表达进行分析。结果子宫内膜癌细胞组织内miR-34a表达量明显低于癌旁组织(P<0.05);与miR-34a mimics组比较,miR-34a mimics联合Notch 1组的细胞活力明显增加(P<0.05);与miR-NC组比较,miR-34a mimics组的细胞凋亡率明显增加,差异有统计学意义(P<0.05);与miR-NC组比较,miR-34a mimics组Ishiknwz细胞内Notch 1表达明显下降,但PARP降解产物表达明显增加(P<0.05);对比miR-34a mimics组,miR-34a mimics联合Notch 1组Ishikawa细胞内Notch 1表达明显升高,但PARP降解产物表达明显下降(P<0.05)。结论miR-34a可对靶基因Notch 1表达进行调控,并对子宫内膜癌细胞组织增殖进行抑制,加快细胞凋亡。

Objective To investigate the expression of miR-34a in endometrial cancer cells and its effect on the proliferation and apoptosis of endometrial cancer cells.Methods Fluorescence quantitative PCR was used to detect the expression of miR-34a in endometrial cancer cell tissues and adjacent tissues.The target gene of miR-34a was determined by dual luciferase reporter gene.Endometrial cancer cells Ishikawa were selected to carry out miR-34a mimics,control miR-NC transfection,and transfection of Notch 1 expression vector;The interference of miR-34a and Notch 1 on the proliferation and apoptosis of endometrial cancer miR-34a cells was analyzed by MTT method and flow cytometry.Then the Western-blot method was used to analyze the interference of miR.-34a with the expression of Notch 1 protein.Results The expression of miR-34a in endometrial cancer cell tissues was significandy lower than that in adjacent tissues(P<0.05).Compared with the miR-34a mimics group,the cell viability of the miR-34a mimics combined with Notch 1 group was significantly increased(P<0.05).Compared with the miR-NC group,the apoptosis rate of the miR-34a mimics group was significantly increased(P<0.05).Compared with the miR-NC group,the expression of Notch 1 in Ishikawa cells of the miR.-34a mimics group was significantly decreased,but the expression of PARP degradation products was significantly increased(P<0.05).Compared with the miR-34a mimics group,the expression of Notch 1 in Ishikawa cells of the miR-34a mimics combined with Notch 1 group was significantly increased,but the expression of PARP degradation products was significantly decreased(P<0.05).Conclusion miR-34a can regulate the expression of target gene Notch 1,inhibit the proliferation of endometrial cancer cells,and accelerate cell apoptosis.