基于生物信息学分析PDE2A基因在消化系统肿瘤预后及免疫浸润的临床意义

Clinical significance of PDE2A in prognosis and immune infiltration in gastrointestinal cancer based on bioinformatics analysis

背景消化系统肿瘤是最常见的恶性肿瘤之一,然而其预后仍不好.探索潜在生物标志物是肿瘤研究的重要方向.我们拟利用生物信息学方法探索消化系统的潜在标志物.目的探究在消化系统肿瘤中PDE2A表达与免疫浸润相关性,探索PDE2A在消化系统肿瘤中的预后价值.方法通过HPA、TIMER和UALCAN数据库分析PDE2A在不同消化系统肿瘤中的mRNA及蛋白表达,利用GEPIA数据库探索PDE2A在不同消化系统肿瘤患者预后的影响.通过TIMER数据库探索PDE2A表达与不同消化系统肿瘤免疫浸润相关性.利用cBioPortal数据库分析PDE2A在不同消化系统肿瘤的遗传突变信息.通过STRING数据库探索PDE2A的潜在蛋白相互作用网络.结果PDE2A基因mRNA和蛋白在肝癌、胃癌、胰腺癌、结肠癌和食管癌组织中表达低于正常癌旁组织.PDE2A高表达的肝癌和胰腺癌患者较PDE2A低表达的患者预后良好.PDE2A表达与肝癌、胃癌、胰腺癌、结肠癌和食管癌不同免疫细胞如CD4+T淋巴细胞、CD8+T淋巴细胞、B细胞、巨噬细胞和中性粒细胞呈正相关或负相关.PDE2A基因在胃癌、胰腺癌、结肠癌及食管癌中存在错义突变等遗传改变.结论PDE2A表达与消化系统肿瘤免疫浸润有关.PDE2A高表达的肝癌和胰腺癌患者预后良好.PDE2A基因可能为潜在的消化系统肿瘤免疫治疗靶点和预后标志物.

BACKGROUND Gastrointestinal cancer is one of the most common malignant cancers,but its prognosis is still poor.Exploring potential biomarkers is an important aspect of cancer research.We intended to explore potential biomarkers for gastrointestinal cancer using bioinformatics methods.AIM To explore the correlation between the expression of PDE2A and immune infiltration in gastrointestinal cancer,and to investigate the prognostic value of PDE2A in gastrointestinal cancer.METHODS The mRNA and protein expression of PDE2A in different gastrointestinal cancers was analyzed based on the HPA,TIMER,and UALCAN databases.GEPIA database was used to investigate the effect of PDE2A on prognosis of patients with different gastrointestinal cancers.The relationship between PDE2A expression and immune infiltration was analyzed using the TIMER database.Frequencies of PDE2A mutations were analyzed using cBioPortal database.Protein–protein interaction network analysis of PDE2A was based on the STRING database.RESULTS The mRNA and protein expression of PDE2A in liver hepatocellular carcinoma(LIHC),stomach adenocarcinoma(STAD),pancreatic adenocarcinoma(PAAD),colon adenocarcinoma(COAD),and esophageal carcinoma(ESCA)was lower than that in normal adjacent tissues.Patients with LIHC and PAAD with high PDE2A expression had a better prognosis.The expression of PDE2A was positively or negatively correlated with different immune cells such as CD4+T cells,CD8+T cells,B cells,macrophages,and neutrophils in LIHC,STAD,PAAD,COAD,and ESCA.Finally,there were missense mutations in PDE2A gene in STAD,PAAD,COAD,and ESCA.CONCLUSION The expression of PDE2A is related to immune infiltration in gastrointestinal cancer.Patients with LIHC and PAAD with high PDE2A expression have a better prognosis.PDE2A may be a potential immunotherapy target and prognostic marker for gastrointestinal cancer.