自组装形成的透明质酸-铜-槲皮素配位聚合物纳米粒子对乳腺癌的协同治疗作用

Synergistic therapeutic effect of self-assembled hyaluronic acid-copper-quercetin coordination polymer nanoparticles on breast cancer

目的:探讨自组装形成的具有协同治疗作用的透明质酸-铜-槲皮素配位聚合物纳米粒子(HCQ NP)对乳腺癌的协同治疗作用。方法:采用自组装方法合成HCQ NP,并通过透射电镜、动态光散射、红外光谱分析和电感耦合等离子体质谱对其进行表征;采用亚甲蓝紫外吸收法测定HCQ NP与不同浓度的过氧化氢(H_(2)O_(2))反应生成羟基自由基(·OH)的能力;在模拟肿瘤微环境下,采用CCK-8法分别评价不同浓度的HCQ NP对乳腺癌MDA-MB-231细胞的抑制效果。结果:自组装形成的HCQ NP具有形状类似球形、大小均一的特点,并对乳腺癌MDA-MB-231细胞具有显著的协同抑制作用。结论:自组装形成的HCQ NP对乳腺癌MDA-MB-231细胞具有协同抑制作用。

Objective:To explore the synergistic therapeutic effect of hyaluronic acid-cu-quercetin coordination polymer nanoparticles(HCQ NP)formed by self-assembly and the inhibitory effect on breast cancer.Methods:HCQ NP was synthesized by self-assembly method and characterized by transmission electron microscopy,dynamic light scattering,infrared spectroscopy and inductively coupled plasma mass spectrometry.The methylene blue ultraviolet absorption method was used to determine the ability of HCQ NP to react with different concentrations of hydrogen peroxide(H_(2)O_(2))to generate hydroxyl radicals(·OH).In the simulated tumor microenvironment,the CCK-8 method was used to evaluate the inhibitory effects of different concentrations of HCQ NP on breast cancer MDA-MB-231 cells.Results:The self-assembled HCQ NPs had the characteristics of similar spherical shape and uniform size,and had a significant synergistic inhibitory effect on breast cancer MDA-MB-231 cells.Conclusion:The self-assembled HCQ NPs have a significant synergistic inhibitory effect on breast cancer MDA-MB-231 cells.