TLR4 mRNA和TCF21 mRNA在喉癌组织中的表达及其与临床病理的相关性

Expression of TLR4 mRNA and TCF21 mRNA in laryngeal carcinoma and their correlation with clinicopathology

目的:探究Toll样受体4(TLR4)、转录因子21(TCF21)在喉癌组织中的表达及其与临床病理的相关性。方法:选择95例喉癌患者的喉癌组织及癌旁正常组织,实时荧光定量PCR技术分别检测喉癌组织及癌旁正常组织中TLR4 mRNA及TCF21 mRNA表达水平,并分析其与临床病理参数之间的相关性。结果:与癌旁正常组织相比,喉癌组织的TLR4 mRNA表达升高(P<0.05),TCF21 mRNA降低(P<0.05)。TLR4 mRNA及TCF21 mRNA表达与癌组织分化程度、浸润深度、淋巴结转移、临床分期的差异均有统计学意义(P<0.05)。癌组织的TLR4 mRNA表达水平与TCF21 mRNA表达呈负相关性(r=-0.454,P<0.001)。结论:TLR4 mRNA高表达和TCF21 mRNA低表达可能促进喉癌细胞恶性增殖及转移,检测喉癌组织TLR4和TCF21 mRNA指标对评估喉癌恶性程度、转移潜能及预后具有积极的指导意义。

Objective:To investigate the expression of Toll like receptor 4(TLR4)and transcription factor 21(TCF21)in laryngeal carcinoma and their correlation with clinicopathological.Methods:95 patients with laryngeal cancer were selected for the study,and their laryngeal cancer tissues and adjacent normal tissues were collected as experimental samples.The expression of TLR4 and tcf21 mRNA was detected by real-time fluorescent quantitative PCR(RT-PCR),and the correlation between the positive expression and clinicopathological parameters was analyzed.Results:The expression level of TLR4 mRNA in laryngeal carcinoma tissues was significantly higher than the adjacent normal tissues(P<0.05),while the expression level of TCF21 mRNA in laryngeal carcinoma tissues was significantly lower than the adjacent normal tissues(P<0.05).In laryngeal carcinoma,the expression of TLR4 mRNA and TCF21 mRNA was significantly different in the degree of differentiation,depth of invasion,lymph node metastasis and clinical stage(P<0.05).The expression of TLR4 and TCF21 was negatively correlated in laryngeal carcinoma(P<0.05).Conclusion:The high expression of TLR4 and low expression of TCF21 may promote the malignant proliferation and metastasis of laryngeal carcinoma cells.The detection of these two indicators has positive guiding significance for evaluating the malignant degree,metastatic potential and prognosis of laryngeal carcinoma.