hsa_circRNA_104633和hsa_circRNA_406281在原发性痛风中的变化及临床意义

Changes and clinical significance of hsa_circRNA_104633 and hsa_circRNA_406281 in primary gout

目的:探讨hsa_circRNA_104633和hsa_circRNA_406281在原发性痛风患者外周血单个核细胞(PBMCs)中的变化及临床意义。方法:收集45例急性期痛风患者(AG组)、45例间歇期痛风患者(IG组)及60例健康体检者(HC组)的外周静脉血标本及临床资料。采用实时荧光定量多聚合酶链反应检测hsa_circRNA_104633和hsa_circRNA_406281在所有研究对象PBMCs中的相对表达量,分析其与痛风患者的临床指标的相关性,并构建受试者工作特征曲线(ROC)评估两个circRNA在痛风中的诊断价值。结果:hsa_circRNA_104633在AG组及IG组的表达均高于HC组(P<0.05),而AG组与IG组比较,差异无统计学意义(P>0.05);hsa_circRNA_406281在AG组的表达高于IG组及HC组(P<0.05),但IG与HC组比较,差异无统计学意义(P>0.05)。相关性分析显示,hsa_circRNA_104633与血尿酸(sUA)水平成正相关(r=0.305,P=0.003),hsa_circRNA_406281与C反应蛋白(CRP)、sUA水平呈负相关(CRP:r=-0.513,P<0.001;sUA:r=-0.213,P=0.043);ROC曲线分析显示,hsa_circRNA_104633用于诊断痛风的曲线下面积为0.822。结论:hsa_circRNA_104633和hsa_circRNA_406281可能参与了痛风患者尿酸代谢调节,hsa_circRNA_104633有望成为痛风诊疗的生物标志物。

Objective:To explore the changes and clinical significance of hsa_circRNA_104633 and hsa_circRNA_406281 in peripheral blood mononuclear cells(PBMCs)of patients with primary gout.Methods:Peripheral venous blood samples and clinical data of 45 patients with acute gout(AG group),45 patients with intercriticalgout(IG group)and 60 healthy subjects(HC group)were collected.Real-time fluorescence quantitative polymerase chain reaction was used to detect the relative expression levels of hsa_circRNA_104633 and hsa_circRNA_406281 in PBMCs of all subjects,and the correlation between them and the clinical indicators of gout patients were analyzed.Receiver operating characteristic curve(ROC)was constructed to evaluate the diagnostic value of two circRNAs in gout.Results:The expression of hsa_circRNA_104633 in the AG and IG groups was significantly higher than that in the HC group(P<0.05),and there was no statistically significant difference between the AG and IG group(P>0.05).The expression of hsa_circRNA_406281 in the AG group was significantly higher than that of IG and HC group(P<0.05),there was no significant difference between IG and HC group(P>0.05).hsa_circRNA_104633 was positively correlated with serum uric acid uSA level(r=0.305,P=0.003).hsa_circRNA_406281 was negatively correlated with C-reactive protein(CRP)and uSA levels(r=-0.513,P<0.001,r=-0.213,P=0.043).ROC curve analysis showed that the area under the curve of hsa_circRNA_104633 for diagnosing gout was 0.822.Conclusion:hsa_circRNA_104633 and hsa_circRNA_406281 may be involved in the regulation of gout uric acid metabolism,and hsa_circRNA_104633 is expected to become a biomarker for the diagnosis and treatment of gout.