正畸力作用下压力侧牙周组织中RIP1、RIP3和MLKL的表达

Expression of RIP1,RIP3 and MLKL in Inflammatory Periodontal Tissue under Orthodontic Force

目的:RIP1、RIP3和MLKL是坏死性凋亡主要的蛋白因子,在引起细胞死亡及炎症反应中起到重要的作用,本实验旨在研究大鼠正畸牙齿移动过程中压力侧牙周组织内RIP1、RIP3和MLKL的表达变化,探索其在压力侧牙周组织改建中的作用。方法:选取30只健康雄性SD大鼠为研究对象,随机分为6组:0 d、1 d、3 d、5 d、7 d、14 d组,每组5只,0 d组为空白对照组,以上颌两中切牙为支抗,镍钛拉簧拉左侧第一磨牙向前,力值为50 g。在各时间点处死大鼠后取其第一磨牙及周围牙周组织,免疫组织化学法检测压力侧RIP1、RIP3和MLKL的表达水平。结果:与空白对照组比较,实验组中RIP1、RIP3和MLKL的表达增加,表达趋势相似均为先升高后下降,但RIP1的表达在3 d、5 d、7 d时增加明显(P<0.05),RIP3的表达在3 d、5 d时增加明显(P<0.05),而MLKL的表达在5 d时增加明显(P<0.05)。结论:正畸牙齿移动过程中压力侧存在坏死性凋亡,RIP1、RIP3和MLKL参与正畸牙齿移动过程中压力侧牙周组织的改建。

Objective:To study the expression of RIP1,RIP3,and MLKL in the pressure-side periodontal tissue during orthodontic tooth movement in rats and to explore its role in pressure-side periodontal tissue remodeling.Methods:Thirty healthy male SD rats were selected as the research object in this experiment and randomly divided into 6 groups:0 d,1 d,3 d,5 d,7 d and 14 d groups,with 5 rats in each group.0 d group was the blank control group.The two central incisors of the upper jaw were anchors,and the left first molar was pulled forward with a force value of 50 g by a nickel-titanium tension spring.After the rats were sacrificed at each time point,the first molar and the surrounding periodontal tissues were collected,and the expression levels of RIP1,RIP3,and MLKL on the pressure side were detected by immunohistochemistry.Results:Compared with the blank control group,the expression of RIP1,RIP3,and MLKL in the experimental group increased at first and then decreased,and the expression at each time point was higher than that in the blank control group.The expression increased significantly on the 7th day.Conclusion:There was necroptosis in the pressure side during the orthodontic tooth movement,and RIP1,RIP3 and MLKL were both involved in the periodontal tissue remodeling on the pressure side during orthodontic tooth movement.