微纳米共存的磷酸化涂层对钛植入体骨结合的影响

Effect of micro/nanoscaled Ti phosphate/Ti oxide hybrid coating on the osseointegration of Ti implants

目的在纯钛植入体表面制备出微纳米共存的具备晶体相"磷"的仿生结构,探究其对钛植入体骨结合的影响。方法利用特殊压强下碱热磷酸反应法在纯钛表面制备出微纳米共存的磷酸化涂层,即钛磷钛(TiP-Ti),选取未处理的光滑纯钛(cp-Ti)作为对照。对材料进行表征分析,并利用体外细胞学技术探究TiP-Ti对大鼠骨髓间充质干细胞(BMSCs)增殖、黏附以及分化的影响。最后,将材料植入大鼠体内,12周后评估TiP-Ti对宿主骨结合的影响。结果 TiP-Ti表面呈现纳米级的3D空间结构与微米级孔隙共存的仿生结构。体外实验显示BMSCs在TiP-Ti表面具有更好的黏附、增殖和成骨向分化。体内实验显示,TiP-Ti诱导了与宿主组织的更牢固的界面结合,极限抗剪切强度和最大推出力显著提高。结论本研究成功构建了TiPTi微纳米共存的仿生结构,促进了植入体的骨结合能力,是一种具有应用前景的植入体表面改性。

Objective This study was performed to fabricate a bionic coating with titanium(Ti) phosphate to promote the osseointegration of Ti substrate implants.Methods Phosphorylated micro/nanocoating was prepared on the surface of pure titanium(i.e.,TiP-Ti) by hydrothermal process under special pressure,and the untreated smooth pure titanium(cp-Ti) was selected as the control.To evaluate the characteristics of the coating surface,scanning electron microscopy,X-ray diffraction,atomic force microscopy,and contact-angle measurement were performed.In addition,the effects of TiP-Ti on the proliferation,adhesion,and differentiation of rat bone marrow mesenchymal stem cells(BMSCs)were investigated by using in vitro cytology.Finally,TiP-Ti implants were implanted into the rat tibia,and the effect of TiP-Ti on the osseointegration in the host was evaluated after 12 weeks.Results The TiP-Ti surface presented a bionic structure with coexisting nanoscale 3 D spatial structure and microscale pores.In vitro experiments showed that the BMSCs had enhanced adhesion,proliferation,and osteogenic differentiation on the TiP-Ti surface.Furthermore,in vivo,TiPTi showed considerably stronger osseointegration compared with pure titanium,and the ultimate shear strength and maximum pushing force were significantly improved.Conclusion A bionic structure with TiP-Ti micro/nanoscale coating was successfully fabricated,indicating a promising method for modifying the surface of implants.