山莨菪碱缓解幼龄鼠缺氧缺血性脑组织形态和功能损伤

Anisodamine relieves hypoxic-ischemic brain damage and neurologic impairment in young rats

目的探讨山茛菪碱在缺氧缺血性脑损伤幼龄鼠脑组织形态和功能损伤中的调控作用。方法50只幼龄大鼠均分为健康对照组、模型组、模型加药组(尾静脉注射山莨菪碱2.5、5、10 mg/kg)共5组。脑组织干湿重法检测脑指数和脑含水率,HE染色观察脑组织病理损伤,TUNEL染色观察脑海马神经元周围组织细胞凋亡情况,Western blot检测脑组织中Bax/Bcl-2、caspase-9、caspase-3、BDNF和NGF蛋白表达水平,RT-PCR检测BDNF和NGF mRNA表达水平,试剂盒检测SOD、MDA和GSH-Px含量。结果与健康对照组比较,模型组幼龄鼠脑组织形态和神经功能损伤严重(P<0.05)。与模型组比较,5 mg/kg和10 mg/kg山茛菪碱组脑指数和脑含水率降低(P<0.05),脑组织病理损伤好转,脑海马神经元周围组织细胞凋亡减少,Bax/Bcl-2、caspase-9、caspase-3表达水平降低(P<0.05),BDNF和NGF表达水平增高(P<0.05),MDA含量降低,SOD和GSH-Px含量增高(P<0.05)。结论山茛菪碱能够缓解缺氧缺血性脑损伤幼龄鼠脑组织形态和功能损伤。

Objective To investigate the regulatory effect of anisodamine on brain injury and neurological damage in young rats with hypoxic-ischemic brain damage.Methods Fifty young rats were divided into five groups(n=10):a healthy control group,a model group,and three model dosing groups(intravenously injected anisodamine for 2.5,5,10 mg/kg).Brain tissue wet and dry weight method was used to detect brain index and brain water content.HE staining was used to observe the pathological damage of brain tissue.TUNEL staining was used to observe the apoptosis of brain tissues around hippocampal neurons.Western blot detects the protein expression levels of Bax/Bcl-2,caspase-9,caspase-3,BDNF and NGF in brain tissue.RT-PCR was used to detect the expression level of BDNF and NGF mRNA,and the kit was used to detect the content of SOD,MDA and GSH-Px.Results Compared with the healthy control group,the brain tissue and nerve function of young rats in the model group were seriously damaged(P<0.05).Compared with the model group,the brain index and brain water content of young rats in the 5 mg/kg and 10 mg/kg anisodamine groups significantly reduced(P<0.05).The pathological damage of the brain tissue significantly improved,and the apoptotic cells around the hippocampal neurons in the brain Apoptosis decreased,Bax/Bcl-2,caspase-9,and caspase-3 expression levels decreased(P<0.05),BDNF and NGF expression levels increased(P<0.05),MDA content decreased,SOD and GSH-Px content increased(P<0.05).Conclusions Anisodamine can alleviate brain damage and nerve function damage in young rats with HIBD.