可疑植物制品中合成大麻素5F-EDMB-PICA的鉴定

Identification of Synthetic Cannabinoid 5F-EDMB-PICA in Suspicious Herbal Products

目的探讨无对照品时可疑植物制品中未知合成大麻素的定性分析策略。方法采用甲醇对植物制品中的合成大麻素进行提取,提取液经旋转蒸发仪浓缩后采用制备液相分离、纯化,得到高纯度合成大麻素制备样品,综合利用气相色谱-质谱法(gas chromatography-mass spectrometry,GC-MS)、超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry,UPLC-QTOF-MS)、核磁共振(nuclear magnetic resonance,NMR)对制备化合物进行结构解析。结果采用制备液相得到10mg高纯度未知样品,采用GC-MS、UPLC-QTOF-MS和NMR进行分析,通过谱图解析,最终确定未知合成大麻素为2-[1-(5-氟戊基)-1H-吲哚-3-甲酰氨基]-3,3-二甲基丁酸乙酯,简称5F-EDMB-PICA。结论本研究建立了采用制备液相从低含量植物制品中提取未知合成大麻素的方法,并综合利用GC-MS、UPLC-QTOF-MS、NMR实现了对未知物结构的解析,这些信息将有助于法庭科学实验室在鉴定实践中鉴定该物质或其他具有类似结构的化合物。

Objective To study the qualitative analysis strategy for unknown synthetic cannabinoid in the suspicious herbal product when no reference substance is available.Methods The synthetic cannabinoid in herbal blend was extracted with methanol.The extract was concentrated by rotary evaporator and separated and purified by preparative liquid chromatography,to obtain high purity synthetic cannabinoid sample.Gas chromatography-mass spectrometry(GC-MS),ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)and nuclear magnetic resonance(NMR)were used to determine the structure of the prepared compound.Results High purity unknown sample(10 mg)was obtained by preparative liquid chromatography.The sample was analyzed by GC-MS,UPLC-TOF-MS and NMR,and through spectrum analysis,the unknown synthetic cannabinoid was determined as 5F-EDMB-PICA.Conclusion The method to extract unknown synthetic cannabinoid from low content herbal products by preparative liquid chromatography was established,and the structure of the unknown sample was identified by comprehensive use of GC-MS,UPLC-QTOF-MS and NMR.The information will assist forensic laboratories in identifying this substance or other compounds with similar structures in their casework.