UPLC-Q-TOF/MS法测定2,6-二叔丁基对甲酚

Determination of 2,6-di-tert-butyl Paracresol by UPLC-Q-TOF/MS

利用超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOF/MS),建立了对2,6-二叔丁基对甲酚测定的方法.采用色谱柱Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm×1.7μm),以水和乙腈(30∶70,v∶v)为流动相等度洗脱,使用ESI-/APCI复合源,在全扫描模式下对2,6-二叔丁基对甲酚进行检测.结果显示,2,6-二叔丁基对甲酚在5~50 mg/L的浓度范围内线性关系良好,检出限和定量限分别为0.2μg/g和0.7μg/g,回收率在95%~100%之间,相对标准偏差小于1%(n=6).该方法已用于饼干中2,6-二叔丁基对甲酚含量的检测.

A method for the determination of 2,6-di-tert-butyl paracresol was developed based on ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS).The column used was Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm×1.7μm),with the mobile phase of water and acetonitrile(30∶70,v∶v)as the mobile phase and the ESI-/APCI composite source was selected in the full scan mode.A good linear relationship for 2,6-di-tert-butyl paracresol was obtained within the concentration range of 5~50 mg/L,with the limit of detection of 0.2μg/g,the limit of quantification of 0.7μg/g,the recovery of 95%~100%,and the relative standard deviation was less than 1%(n=6).This method has been used to measure the content of BHT in cookies.