妊娠糖尿病Hcy与叶酸代谢基因MTHFRC677T多态性的相关性研究

Association between Hcy and folate metabolism gene MTHFR C677T polymorphism in gestational diabetes mellitus

目的探讨妊娠糖尿病(GDM)叶酸代谢基因MTHFRC677T多态性分布特点及其与血清Hcy水平的相关性。方法选取2018年12月至2020年6月银川市妇幼保健院内科及产科确诊的83例GDM患者(GDM组)及115例健康妊娠妇女(对照组)作为研究对象。比较两组MTHFRC677T基因型及等位基因频率,比较两组不同MTHERC677T基因型血清Hcy水平,采用Logistic模型评估血清Hcy水平和MTHFR C677T基因型发生GDM的风险因素。结果GDM组血清Hcy水平显著高于对照组,差异具有统计学意义(P<0.05);两组MTHFRC677T基因型和等位基因频率比较,差异具有统计学意义(P<0.05),其中TT基因型GDM的发病率(21/30)显著高于CC型(34/93)和CT型(28/75),等位基因T基因GDM的发病率(70/135)显著高于C型(96/121);同一基因型血清Hcy水平比较,TT基因型GDM组血清Hcy水平显著高于对照组,差异具有统计学意义(P<0.05);GDM组不同基因型血清Hcy水平比较,TT基因型血清Hcy水平为(11.4±2.9)μmol/L,显著高于CT型(6.9±1.8)μmol/L和CC型(5.9±2.1)μmol/L,差异具有统计学意义(P<0.05);Logistic分析显示,血清Hcy水平和TT基因型均为发生GDM的独立危险因素。结论MTHERC677T基因多态性可能通过影响血清Hcy水平进而影响GDM的发生、发展。

Objective To investigate the distribution of folic acid metabolism gene MTHFR C677 T polymorphism in gestational diabetes mellitus(GDM)and its correlation with serum Hcy level.Methods 83 GDM patients(GDM group)and 115 healthy pregnant women(control group)diagnosed in Yinchuan Maternal and Child Health Hospital from December 2018 to June 2020 were selected as the research subjects.The MTHFR C677 T genotype and allele frequency,and the serum Hcy level of different MTHER C677 T genotype were compared between the two groups.The Logistic model was used to evaluate the serum Hcy level and the risk factors for GDM of MTHFR C677 T genotype.Results The serum Hcy level in GDM group was significantly higher than that in control group(P<0.05).C677 T genotype and allele frequency of MTHFR were significantly different between the two groups,The incidence of GDM in TT genotype(21/30)was significantly higher than that in CC genotype(34/93)and CT genotype(28/75),and the incidence of GDM in T allele(70/135)was significantly higher than that in C genotype(96/121).The serum Hcy level in the TT genotype GDM group was significantly higher than that in the control group(P<0.05).Comparison of serum Hcy level between different genotypes in GDM group,TT genotype(11.4±2.9)μmol/L was significantly higher than CT type(6.9±1.8)μmol/L and CC type(5.9±2.1)μmol/L(P<0.05).Logistic analysis showed that serum Hcy level and TT genotype were independent risk factors for GDM.Conclusions MTHFR C677 T gene polymorphism may affect the occurrence and development of GDM by influencing Hcy level.