游泳池水中尿素检测方法改进与研究

Improvement and research of urea detection method in swimming pool water

目的作为游泳池水是否合格的评价指标之一,尿素浓度可以表明游泳池水受到人体污染程度。为满足大批量样品检测要求,建立线性范围更宽,实验效率更高,同时结果较为准确和精密的游泳池水中尿素的检测方法。方法采用60孔多孔电热消化器电加热反应体系,改变加热方式,减小取样体积和显色剂体积,选取合适的实验条件,进行实验。结果通过优化反应体系、改变加热方式、考察显色剂使用量、优化显色时间等实验方法的改进,缩短了反应时间,扩大了线性范围,提高了实验效率。结论国家标准方法检测沈阳市2019年、2020年游泳池水尿素发现,部分游泳池水的尿素含量大幅超出了3.5 mg/L的国家限值。本研究方法改进后可满足实验的要求,扩大了国家标准方法检测区间,减少了超标样品重做次数。操作简单、快速,能满足实验室大批量尿素样品的测定。

Objective As one of the evaluation indexes of whether the swimming pool water was qualified or not,the concentration of urea could indicate the pollution degree of swimming pool water by human body.In order to meet the requirements of mass sample detection,a detection method of urea in swimming pool water with wider linear range,higher experimental efficiency and more accurate and precise results was established.Methods The experiments were carried out on using 60 hole porous electrothermal digester heating reaction system,changing heating mode,reducing sampling volume and color reagent volume,selecting suitable experimental conditions.Results Through optimizing the reaction system,changing the heating method,investigating the using volume of color reagent and optimizing the color reagent reaction time,the reaction time was shortened,the linear range was expanded and the experimental efficiency was improved.Conclusion According to the national standard method,the urea concentrations of swimming pool water in Shenyang in 2019 and 2020 were detected.It was found that the urea concentrations in some swimming pool water exceeded the national limit of 3.5 mg/L.The improved method of this research could still meet the requirements of the experiment.The detection range of the national standard method was expanded,and the repeat times of over standard samples were reduced.The operation was simple and fast,which could meet the requirements of the determination of large quantities of urea samples in the laboratory,and reduce the experimental cost.