环指蛋白180、p53及Ki-67在肾透明细胞癌中的表达及临床意义

Expression and clinical significance of the ring finger protein 180,p53 and Ki-67in renal clear cell carcinoma

目的研究环指蛋白180(RNF180)、p53及细胞增殖抗原标记物Ki-67在肾透明细胞癌(RCCC)组织中的表达水平及临床病理意义。方法收集225例确诊RCCC患者术后的石蜡标本及相应的远端正常组织制备组织芯片,用免疫组化法检测RNF180,p53和Ki-67在RCCC组织及癌旁组织的表达。结果在癌组织和癌旁组织中RNF180蛋白的阳性表达率分别为48.89%(110例/225例)和75.56%(170例/225例),p53蛋白的阳性表达率分别为45.71%(96例/210例)和2.38%(5例/210例),Ki-67蛋白的阳性表达率分别为32.20%(66例/205例)和2.44%(5例/205例),差异均有统计学意义(均P<0.05)。Spearman相关性分析显示,RNF180与p53及Ki-67均无相关性。Kaplan-Meier分析显示,RCCC患者的良好预后与RNF180的阳性表达呈正相关(P<0.05),而与p53及Ki-67无关。多因素Cox回归分析显示,TNM分期、肿瘤大小及RNF180是RCCC的独立预后因子。结论RNF180、p53及Ki-67可能分别参与RCCC的发生、发展过程,且RNF180可为RCCC患者预后提供参考。

Objective To explore the expression and clinicopathological significance of tumor suppressor gene ring finger protein 180(RNF180),p53 and Ki-67 in renal clear cell carcinoma(RCCC).Methods Tissue microarray was made from paraffin specimens and corresponding distal normal tissues from 225 patients with RCCC after operation.The expression of RNF180,p53 and Ki-67 in RCCC and paracancerous tissues was detected by immunohistochemical method.Results In cancer tissues and adjacent tissues,the positive expression rates of RNF180 protein were 48.89%(110 cases/225 cases)and 75.56%(170 cases/225 cases),the positive expression rates of p53 protein were 45.71%(96 cases/210 cases)and 2.38%(5 cases/210 cases),the positive expression rates of Ki-67 protein were 32.20%(6 cases/205 cases)and 2.44%(5 cases/205 cases),the differences were statistically significant(all P<0.05).Spearman correlation analysis showed that there was no correlation between RNF180 and p53 and Ki-67.Kaplan-Meier analysis showed that the good prognosis of RCCC patients was positively correlated with the positive expression of RNF180,but not with p53 and Ki-67.Multivariate Cox regression analysis showed that TNM stage,tumor size and RNF180 were independent prognostic factors of RCCC.Conclusion RNF180,p53 and Ki-67 may be involved in the occurrence and development of RCCC,and RNF180 can provide reference for the prognosis of RCCC patients.