狮头鹅 AR 基因克隆和组织表达

Cloning and tissue expression of AR gene in Shitou goose

本研究旨在克隆鹅雄激素受体基因(Androgen receptor,AR),并了解其在不同组织中的表达情况.以狮头鹅为试验材料,采集下丘脑和睾丸组织样品,提取RNA逆转录后进行AR基因克隆,并用RACE扩增其cDNA全长序列,其它同采用实时荧光定量PCR的方法检测AR基因在各个组织中的表达情况.结果克隆获得AR基因全长cDNA序列,共得到4个转录本.通过氨基酸序列同源进行分析,发现狮头鹅AR基因在禽类和哺乳类动物中同源性较高,说明该基因在禽类和哺乳类进化保守.荧光定量PCR结果显示AR基因在狮头鹅12个组织中均有表达量,其中在腿肌、胸肌和睾丸表达量较高,表明AR基因可能参与调控狮头鹅公鹅的肌肉生长与繁殖.

The aim of this study was to clone goose androgen receptor(AR)gene and understand its expression in different tissues.Shitou goose was used as experimental material,hypothalamus and testis tissue were collected for RNA extraction and reverse transcription,the AR gene was clone and RACE was used amplify its full-length cDNA sequence.Real-time fluorescence quantitative PCR(qPCR)was used to detect the expression of AR gene in various tissues.The full-length cDNA sequence of the AR gene was cloned and 4 transcripts were obtained.The amino acid sequence homology analysis showed that AR gene of Shitou goose had high homology in birds and mammals,which indicated that AR gene was evolutionarily conserved in birds and mammals.The results of qPCR showed that AR gene was expressed in all 12 tissues of Shitou geese,among which the expression level was higher in leg muscles,breast muscle and testis,indicating that AR gene may be involved in regulating muscle growth and reproduction of male geese.